Laurenzi M A, Link H
Acta Neurol Scand. 1978 Sep;58(3):141-7. doi: 10.1111/j.1600-0404.1978.tb02873.x.
The mobility of the immunoglobulins G, A and M, beta-trace protein and gramma-trace protein on isoleectric focusing of serum and CSF was determined by immunofixation using specific antisera. Polyclonal IgG migrated as multiple bands between pH 4.7--8.6, polyclonal IgA as multiple bands between pH 4.9--6.1 in CSF and serum. IgM could not be identified in normal CSF or serum. beta-trace protein gave three bands at pH 8.0, 8.4 and 7.4--7.5, respectively, while gamma-trace protein gave one single band at pH 9.5--greater than 9.5. Oligoclonal IgG in CSF in multiple sclerosis and neurosyphilis migrated between pH 8.6--greater than 9.5 and was easily discriminated from other proteins.
通过使用特异性抗血清进行免疫固定，测定了血清和脑脊液在等电聚焦时免疫球蛋白G、A和M、β-微量蛋白和γ-微量蛋白的迁移情况。多克隆IgG在pH 4.7 - 8.6之间迁移为多条带，多克隆IgA在脑脊液和血清中于pH 4.9 - 6.1之间迁移为多条带。在正常脑脊液或血清中未检测到IgM。β-微量蛋白分别在pH 8.0、8.4和7.4 - 7.5处出现三条带，而γ-微量蛋白在pH 9.5 - 大于9.5处出现一条单带。多发性硬化症和神经梅毒患者脑脊液中的寡克隆IgG在pH 8.6 - 大于9.5之间迁移，且易于与其他蛋白质区分开来。
