Kocianová E, Kovácová E I, Literák I
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Folia Parasitol (Praha). 2001;48(3):235-9.
Laboratory animals (mice and guinea pigs) were infected with the isolates of Coxiella burnetii (Derrick, 1939) obtained from bovine milk (M18 and M35) and the ticks Ixodes ricinus (Linnaeus, 1758) and Dermacentor marginatus (Sulzer, 1776) (Kl3 and Kl6, respectively), and with the reference strain Nine Mile. Neither mortality nor lethality occurred with the mice. Antibody response in mice infected with isolates from milk was lower (1:16-512) than that from ticks (1:32-4096). Onset of seropositivity also occurred later - on the 10th day post-infection (p.i.) for M18 and M35 in comparison with the 7th day for Kl3 and Kl6. In guinea pigs, infection manifested by fever. The fever was less evident in guinea pigs infected with isolates from milk (39.5-40.1 degrees C) than in guinea pigs infected with isolates from ticks (39.5-40.6 degrees C). Partially engorged females of Dermacentor reticulatus (Fabricius, 1794) were inoculated with isolates M18 and Kl3. No differences in the multiplication of C. burnetii in haemocytes between these two isolates were ascertained.
将实验室动物（小鼠和豚鼠）用从牛乳中分离得到的伯纳特立克次体（德里克，1939年）（M18和M35）以及蓖麻硬蜱（林奈，1758年）和边缘革蜱（苏尔策，1776年）（分别为Kl3和Kl6）的分离株，还有参考菌株九里株进行感染。小鼠未出现死亡或致死情况。感染牛乳分离株的小鼠抗体反应（1:16 - 512）低于感染蜱分离株的小鼠（1:32 - 4096）。血清阳性的出现时间也较晚——M18和M35在感染后第10天出现血清阳性，而Kl3和Kl6在感染后第7天出现。在豚鼠中，感染表现为发热。感染牛乳分离株的豚鼠发热程度（39.5 - 40.1摄氏度）低于感染蜱分离株的豚鼠（39.5 - 40.6摄氏度）。用分离株M18和Kl3对部分饱血的网纹革蜱（法布尔，1794年）雌蜱进行接种。未确定这两种分离株在血细胞中伯纳特立克次体增殖情况的差异。
