Murga R, Forster T S, Brown E, Pruckler J M, Fields B S, Donlan R M
Epidemiology and Laboratory Branch, Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Microbiology (Reading). 2001 Nov;147(Pt 11):3121-6. doi: 10.1099/00221287-147-11-3121.
Legionellae can infect and multiply intracellularly in both human phagocytic cells and protozoa. Growth of legionellae in the absence of protozoa has been documented only on complex laboratory media. The hypothesis upon which this study was based was that biofilm matrices, known to provide a habitat and a gradient of nutrients, might allow the survival and multiplication of legionellae outside a host cell. This study determined whether Legionella pneumophila can colonize and grow in biofilms with and without an association with Hartmannella vermiformis. The laboratory model used a rotating disc reactor at a retention time of 6.7 h to grow biofilms on stainless steel coupons. The biofilm was composed of Pseudomonas aeruginosa, Klebsiella pneumoniae and a Flavobacterium sp. The levels of L. pneumophila cells present in the biofilm were monitored for 15 d, with and without the presence of H. vermiformis, and it was found that, although unable to replicate in the absence of H. vermiformis, L. pneumophila was able to persist.
军团菌可在人类吞噬细胞和原生动物细胞内感染并繁殖。仅在复杂的实验室培养基上记录到军团菌在无原生动物的情况下生长。本研究基于的假设是，已知能提供栖息地和营养梯度的生物膜基质可能使军团菌在宿主细胞外存活和繁殖。本研究确定了嗜肺军团菌能否在与蠕形哈特曼氏阿米巴相关或不相关的生物膜中定殖和生长。实验室模型使用旋转盘式反应器，保留时间为6.7小时，以在不锈钢试片上生长生物膜。该生物膜由铜绿假单胞菌、肺炎克雷伯菌和一种黄杆菌组成。在有和没有蠕形哈特曼氏阿米巴存在的情况下，对生物膜中嗜肺军团菌细胞的水平进行了15天的监测，发现尽管嗜肺军团菌在没有蠕形哈特曼氏阿米巴的情况下无法复制，但它能够持续存在。
