Cui L, Holmes D, Morgan H
Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, UK.
Electrophoresis. 2001 Oct;22(18):3893-901. doi: 10.1002/1522-2683(200110)22:18<3893::AID-ELPS3893>3.0.CO;2-2.
A linear travelling wave dielectrophoretic (twDEP) microchip was fabricated and used to investigate both the levitation and the twDEP motion of latex beads as a function of applied potential and frequency, suspending medium conductivity, bead size, and surface characteristics. The surface conductance of the latex beads was characterised by measurement of the dielectrophoretic (DEP) crossover frequency. Collection of sample prior to initiation of twDEP was achieved using positive DEP forces generated by an integrated pair of parallel electrodes positioned in front of the twDEP array within the microfluidic channel. The principle of linear twDEP separation is shown using latex beads and rabbit heart cells.
制作了一种线性行波介电泳（twDEP）微芯片，并用于研究乳胶珠的悬浮和twDEP运动，这些运动是施加电势和频率、悬浮介质电导率、珠子尺寸以及表面特性的函数。通过测量介电泳（DEP）交叉频率来表征乳胶珠的表面电导。在twDEP启动之前，利用微流体通道内twDEP阵列前方集成的一对平行电极产生的正DEP力来实现样品收集。使用乳胶珠和兔心脏细胞展示了线性twDEP分离的原理。
