Van Eenoo P, Delbeke F T, de Jong F H, De Backer P
IOC accredited laboratory, Department of Pharmacology, Pharmacy and Toxicology, University Gent, Salisburylaan 133, B-9820, Merelbeke, Belgium.
J Steroid Biochem Mol Biol. 2001 Oct;78(4):351-7. doi: 10.1016/s0960-0760(01)00112-1.
Recently the use of high resolution mass spectrometry or tandem mass spectrometry has enabled the detection of low amounts of anabolic steroids. As a consequence, the post-administration detection time of these drugs has been extended. Recent investigations have shown that norandrosterone, previously unequivocally regarded as evidence of nandrolone administration, might be an endogenous steroid present in small amounts in urine of humans. In this study, very low concentrations (<1 ng/ml) of norandrosterone in urine of a female athlete were detected using tandem mass spectrometry. The presence of norandrosterone was strongly correlated with high plasma 17beta-estradiol levels during the menstrual cycle. Analysis of urine samples from pregnant women supports the hypothesis of formation of precursors for urinary 19-norandrosterone during aromatization of androgens to estrogens. The detection of low urinary concentrations of norandrosterone (0.2-0.5 ng/ml) in samples after strenuous exercise could be regarded as an additional evidence for the existence of such a pathway.
最近，高分辨率质谱或串联质谱的使用使得能够检测到少量的合成代谢类固醇。因此，这些药物给药后的检测时间得以延长。最近的研究表明，以前被明确视为诺龙给药证据的去甲雄酮，可能是人体尿液中少量存在的内源性类固醇。在本研究中，使用串联质谱法在一名女运动员的尿液中检测到了极低浓度（<1纳克/毫升）的去甲雄酮。在月经周期中，去甲雄酮的存在与高血浆17β-雌二醇水平密切相关。对孕妇尿液样本的分析支持了雄激素芳香化转化为雌激素过程中形成尿液19-去甲雄酮前体的假说。在剧烈运动后的样本中检测到低尿液浓度的去甲雄酮（0.2-0.5纳克/毫升）可被视为存在这种途径的额外证据。
