Dahr W, Uhlenbruck G, Bird G W
Vox Sang. 1975;28(2):133-48. doi: 10.1111/j.1423-0410.1975.tb02751.x.
The nature of the receptor sites for several agglutinins is characterized by hemagglutination inhibition assays. The inhibitory activity of human erythrocytes glycoproteins, from which sialic acid, sialic acid and galactose or alkali-labile oligosaccharides have been removed, is compared to the inhibitory effect of compounds with known structure. It is shown that the lectin from Arachis hypogea and anti-T bind to alkali-labile galactosyl-residues. Agglutinins from Bauhinia purpurea and variegata (non- or N-specific), Maclura aurantiaca, Iberis amara, sempervirens, umbellata hybrida and umbellata nana (M- or nonspecific), Moluccella laevis (A- plus N-specific), Helix pomatia, Helix aspersa, Helix lucorum and Caucasotachea atrolabiata interact with alkali-labile N-acetylgalactosamine. The results obtained with the anti-A agglutinins from various snails suggest that human erythrocyte glycoproteins contain, besides the alkali-labile tetrasaccharide, a peptide-linked sialyl-N-acetyl-galactosaminyl-residue. The investigations do not allow a precise definition of the receptor sites for the lectins having M- or N-specificity.
几种凝集素的受体位点性质通过血凝抑制试验来表征。将去除了唾液酸、唾液酸和半乳糖或碱不稳定寡糖的人红细胞糖蛋白的抑制活性与具有已知结构的化合物的抑制作用进行比较。结果表明，花生凝集素和抗 -T 凝集素与碱不稳定的半乳糖基残基结合。紫羊蹄甲和洋紫荆（非特异性或 N 特异性）、桑橙、屈曲花、常绿屈曲花、杂种伞形屈曲花和矮伞形屈曲花（M 特异性或非特异性）、假荆芥、几种蜗牛（A 加 N 特异性）、罗马蜗牛、花园蜗牛、玛瑙螺和高加索嗜螺的凝集素与碱不稳定的 N - 乙酰半乳糖胺相互作用。从各种蜗牛获得的抗 -A 凝集素的结果表明，除了碱不稳定的四糖外，人红细胞糖蛋白还含有肽连接的唾液酸 -N - 乙酰半乳糖胺基残基。这些研究无法精确界定具有 M 或 N 特异性凝集素的受体位点。
