通过逆转录巢式聚合酶链反应诊断奥罗普切病毒感染
Diagnosis of Oropouche virus infection by RT-nested-PCR.
作者信息
Moreli Marcos Lázaro, Aquino Victor Hugo, Cruz Ana Cecília R, Figueiredo Luiz Tadeu M
机构信息
Virus Research Unit, School of Medicine of Ribeirão Preto, University of São Paulo, Campus da USP, Ribeirão Preto, SP, Brazil.
出版信息
J Med Virol. 2002 Jan;66(1):139-42. doi: 10.1002/jmv.2122.
Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.
使用逆转录聚合酶链反应（RT-PCR），其引物可与布尼亚病毒基因组片段的5'和3'末端退火，以及在巢式PCR中与辛布血清群病毒S片段退火的内部引物，能够从三名患者的血清中扩增出奥罗普切病毒（ORO）基因组。这些结果表明，这种逆转录巢式PCR是快速诊断奥罗普切热感染的有用工具。
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