da Fonseca Flávio G, Trindade Giliane S, Silva Ricardo L A, Bonjardim Cláudio A, Ferreira Paulo C P, Kroon Erna G
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, caixa postal 2496, cep: 31270-901, Belo Horizonte, MG, Brazil1.
J Gen Virol. 2002 Jan;83(Pt 1):223-228. doi: 10.1099/0022-1317-83-1-223.
The SPAn232 virus (SPAnv) was isolated from sentinel mice in the forest of Cotia, São Paulo, Brazil. It was grouped originally as a Cotia virus (CV) sample due to serological cross-reaction with the latter. However, SPAnv presented genetic characteristics that differed from CV and indicated that SPAnv is a member of the vaccinia virus (VV) subgroup. SPAnv showed a HindIII-digested DNA pattern similar to those of the WR and Lister strains of VV. Also, SPAnv presented genes homologous to the vaccinia growth factor, thymidine kinase and A-type inclusion (ATI) genes from VV. RFLP analysis of the SPAnv ATI homologous gene indicated that the virus belongs to the VV group. Nucleotide sequences from SPAnv genes showed up to 99% similarity with the same genes from VV. Such a relationship was confirmed visually through the drawing of phylogenetic trees. The results point out the occurrence of a VV strain that is possibly in active circulation in the forests of Southeast Brazil.
SPAn232病毒（SPAnv）是从巴西圣保罗科蒂亚森林中的哨兵小鼠身上分离出来的。由于与科蒂亚病毒（CV）存在血清学交叉反应，它最初被归类为CV样本。然而，SPAnv呈现出与CV不同的遗传特征，表明SPAnv是痘苗病毒（VV）亚组的成员。SPAnv显示出与VV的WR和李斯特菌株相似的经HindIII消化的DNA模式。此外，SPAnv具有与VV的痘苗生长因子、胸苷激酶和A型包涵体（ATI）基因同源的基因。对SPAnv ATI同源基因的RFLP分析表明该病毒属于VV组。SPAnv基因的核苷酸序列与VV相同基因的相似度高达99%。通过绘制系统发育树直观地证实了这种关系。结果指出在巴西东南部森林中可能存在一种正在活跃传播的VV毒株。
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