Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Emerg Infect Dis. 2010 Jun;16(6):976-9. doi: 10.3201/eid1606.091187.
To detect orthopoxvirus in the Brazilian Amazon, we conducted a serosurvey of 344 wild animals. Neutralizing antibodies against orthopoxvirus were detected by plaque-reduction neutralizing tests in 84 serum samples. Amplicons from 6 monkey samples were sequenced. These amplicons identified vaccinia virus genetically similar to strains from bovine vaccinia outbreaks in Brazil.
为了在巴西亚马孙地区检测正痘病毒，我们对 344 种野生动物进行了血清学调查。通过蚀斑减少中和试验，在 84 份血清样本中检测到了针对正痘病毒的中和抗体。对 6 份猴子样本的扩增子进行了测序。这些扩增子鉴定出的牛痘病毒与巴西牛痘暴发的毒株在基因上相似。
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