Humann-Ziehank E, Bickhardt K
Clinic for Pigs and Small Ruminants, Forensic Medicine and Ambulatory Service, School of Veterinary Medicine, Hannover, Germany.
J Vet Med A Physiol Pathol Clin Med. 2001 Nov;48(9):537-44. doi: 10.1046/j.1439-0442.2001.00382.x.
The effects of a single oral application of D-penicillamine (DPA, mean dosage 28 mg/kg body weight) on urinary copper (Cu) excretion and general renal function in six high-Cu supplemented sheep (Cu intake of 3.7 mg/day per kg body weight for 84 days) and four controls (Cu intake of 0.16 mg/day per kg body weight) were investigated to quantify induced cupruresis and the therapeutic effect of DPA as a decoppering agent. Changes in liver Cu concentration were examined before and after DPA treatment by liver biopsies. The influence of DPA treatment on general renal function was low. A 10-fold increase in renal Cu excretion was induced in both groups of sheep. Maximal Cu excretion was observed 4 h after DPA treatment, with mean values of 280 pmol/min per kg body weight in the high Cu group and 145 pmol/min per kg body weight in the controls. In the high Cu sheep, urinary Cu excretion within 24 h after DPA application was equivalent to only 0.42 +/- 0.26% of liver Cu content (mean concentration 347 +/- 124 mg/kg wet weight). Moreover, no effect of DPA on liver Cu concentration was evident. These findings demonstrate that a single application of DPA is not effective in inducing sufficient Cu loss from the bodies of Cu-loaded sheep.
研究了单次口服 D-青霉胺（DPA，平均剂量 28 mg/kg 体重）对六只高铜补充绵羊（84 天内铜摄入量为 3.7 mg/天·kg 体重）和四只对照绵羊（铜摄入量为 0.16 mg/天·kg 体重）尿铜（Cu）排泄及一般肾功能的影响，以量化诱导的尿铜增多以及 DPA 作为排铜剂的治疗效果。通过肝活检检查 DPA 治疗前后肝脏铜浓度的变化。DPA 治疗对一般肾功能的影响较小。两组绵羊的肾铜排泄均增加了 10 倍。DPA 治疗后 4 小时观察到最大铜排泄量，高铜组的平均值为 280 pmol/分钟·kg 体重，对照组为 145 pmol/分钟·kg 体重。在高铜绵羊中，DPA 应用后 24 小时内的尿铜排泄量仅相当于肝脏铜含量的 0.42±0.26%（平均浓度 347±124 mg/kg 湿重）。此外，DPA 对肝脏铜浓度没有明显影响。这些发现表明，单次应用 DPA 对从高铜绵羊体内诱导足够的铜流失无效。
