Gooneratne S R, Christensen D A
Animal and Veterinary Sciences Group, PO Box 84, Lincoln University, Canterbury, New Zealand.
Vet J. 1997 Mar;153(2):171-8. doi: 10.1016/s1090-0233(97)80037-8.
The effect of tetrathiomolybdate (TM), disodium calcium ethylenediamine tetraacetate (EDTA), D-penicillamine (PEN), 2-3 dimercapto-1-propanol (BAL) and dimethyl dithiocarbamate (DDC) administration on biliary and urinary excretion of copper (Cu), zinc (Zn) and iron (Fe) was investigated in sheep on a low Cu diet (Group A) and a high Cu diet (Group B). Only biliary Cu excretion increased significantly (P<0.01) with TM treatment. Urinary Cu excretion increased (P<0.01) following PEN treatment. TM, EDTA, PEN, BAL and DDC adminstration increased Cu excretion via bile and urine by 254, 11, 266, 46 and 16%, respectively in Group A sheep, and by 354, 13, 196, 20 and (-) 31% in Group B sheep. Urinary Zn excretion increased (P<0.01) following EDTA and PEN treatments. Only urinary Fe excretion increased (P<0.01) with EDTA treatment. The results show that TM and PEN are the most efficient decoppering agents, but PEN unlike TM also removes Zn. The major routes of excretion of Cu chelates by TM and PEN are different. TM increases Cu excretion significantly (P<0.05 in Group A and P<0.01 in Group B) in bile with only a slight increase in urinary Cu whereas PEN increases Cu excretion significantly (P<0.01) in urine. Therefore from a therapeutic view, a combination therapy of TM and PEN would be useful to maximize Cu removal from the body.
在低铜饮食（A组）和高铜饮食（B组）的绵羊中，研究了给予四硫代钼酸盐（TM）、乙二胺四乙酸二钠钙（EDTA）、D-青霉胺（PEN）、2-3-二巯基-1-丙醇（BAL）和二甲基二硫代氨基甲酸盐（DDC）对铜（Cu）、锌（Zn）和铁（Fe）胆汁及尿液排泄的影响。仅TM处理使胆汁铜排泄显著增加（P<0.01）。PEN处理后尿液铜排泄增加（P<0.01）。在A组绵羊中，TM、EDTA、PEN、BAL和DDC处理分别使通过胆汁和尿液的铜排泄增加254%、11%、266%、46%和16%，在B组绵羊中分别增加354%、13%、196%、20%和（-）31%。EDTA和PEN处理后尿液锌排泄增加（P<0.01）。仅EDTA处理使尿液铁排泄增加（P<0.01）。结果表明，TM和PEN是最有效的排铜剂，但与TM不同，PEN还会去除锌。TM和PEN排出铜螯合物的主要途径不同。TM使胆汁中的铜排泄显著增加（A组P<0.05，B组P<0.01），而尿液中的铜仅略有增加，而PEN使尿液中的铜排泄显著增加（P<0.01）。因此，从治疗角度来看，TM和PEN联合治疗对于最大限度地从体内去除铜将是有用的。
