Matsusaki Michiya, Serizawa Takeshi, Kishida Akio, Endo Takeshi, Akashi Mitsuru
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, I-21-40 Korimoto, Kagoshima 890-0065, Japan.
Bioconjug Chem. 2002 Jan-Feb;13(1):23-8. doi: 10.1021/bc010008d.
gamma-Poly(glutamic acid) (gamma-PGA), which is produced by Bacillus subtilis, was sulfonated using 2-aminoethane-1-sulfonic acid (taurine) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (WSC) to give sulfonated gamma-PGA (gamma-PGA-sulfonate). From (1)H NMR spectroscopy and IR spectroscopy, it was confirmed that taurine was introduced to the side chain of gamma-PGA via an amide linkage. By altering the synthetic conditions, it was possible to control the content of sulfonate in gamma-PGA-sulfonate. Anticoagulant activity was investigated in order to evaluate the biological activity of gamma-PGA-sulfonate by the Lee-White test. The clotting time was prolonged when the concentration of gamma-PGA-sulfonate on the degree of sulfonation was increased. It becomes clear that gamma-PGA-sulfonate is potentially useful for various medical applications, such as drug delivery, tissue engineering, and medical materials.
γ-聚谷氨酸（γ-PGA）由枯草芽孢杆菌产生，在1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（WSC）存在下，使用2-氨基乙烷-1-磺酸（牛磺酸）对其进行磺化，得到磺化γ-聚谷氨酸（γ-PGA-磺酸盐）。通过核磁共振氢谱（¹H NMR）和红外光谱（IR）证实，牛磺酸通过酰胺键引入到γ-聚谷氨酸的侧链上。通过改变合成条件，可以控制γ-PGA-磺酸盐中磺酸盐的含量。为了通过Lee-White试验评估γ-PGA-磺酸盐的生物活性，对其抗凝活性进行了研究。当γ-PGA-磺酸盐浓度和磺化程度增加时，凝血时间延长。γ-PGA-磺酸盐在药物递送、组织工程和医疗材料等各种医学应用中具有潜在用途，这一点变得清晰起来。
