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炭疽芽孢杆菌和蜡样芽孢杆菌中利福平耐药性的分子分析。

Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus.

作者信息

Vogler Amy J, Busch Joseph D, Percy-Fine Stephanie, Tipton-Hunton Christine, Smith Kimothy L, Keim Paul

机构信息

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.

出版信息

Antimicrob Agents Chemother. 2002 Feb;46(2):511-3. doi: 10.1128/AAC.46.2.511-513.2002.

DOI:10.1128/AAC.46.2.511-513.2002
PMID:11796364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC127050/
Abstract

Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 x 10(-9) mutations/generation by a Luria-Delbrück fluctuation test.

摘要

从经紫外线处理的蜡样芽孢杆菌（20个突变体）和减毒炭疽芽孢杆菌（23个突变体）中筛选出利福平抗性突变体。此外，在减毒炭疽芽孢杆菌中也分离出了自发利福平抗性突变体（22个突变体）。对所有65个突变体的rpoB基因的利福平抗性簇进行了测序。与抗性相关的突变与其他细菌的突变一致，不过观察到了两个新的变化。通过Luria-Delbrück波动试验估计抗性的自发率为1.57×10⁻⁹个突变/代。

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