Khokhar S, Magnusdottir S G M
Procter Department of Food Science, University of Leeds, Leeds LS2 9JT, United Kingdom.
J Agric Food Chem. 2002 Jan 30;50(3):565-70. doi: 10.1021/jf010153l.
Levels of total phenol, catechins, and caffeine in teas commonly consumed in the United Kingdom have been determined using reversed phase high-performance liquid chromatography. Tea bags or tea leaves were purchased from local supermarkets and extracted in boiling water for 5 min. The resulting data showed considerable variability in both total phenols [80.5-134.9 mg/g of dry matter (DM) in black teas and 87-106.2 mg/g of DM in green teas] and catechins (5.6-47.5, 51.5-84.3, and 8.5-13.9 mg/g of DM in black, green, and fruit teas, respectively); this was most probably a result of differing agronomic conditions, leaf age, and storage during and after transport, as well as the degree of fermentation. Caffeine contents of black teas (22-28 mg/g of DM) were significantly higher than in less fermented green teas (11-20 mg/g of DM). The relative concentration of the five major tea catechins ranked EGCG > ECG > EC > EGC > C. The estimated U.K. dietary intakes of total tea catechins, calculated on the basis of an average tea consumption of three cups of tea (200 mL cup, 1% tea leaves w/v), were 61.5, 92.7, and 405.5 mg/day from fruit teas, black teas, and green teas, respectively. The coefficients of variation were 19.4, 88.6, and 17.3%, respectively, indicating the wide variation in these intakes. The calculated caffeine intake ranged between 92 and 146 mg/day. In addition, many individuals will consume much larger quantities of tea, of various strengths (as determined by the brewing conditions employed). This broad spread of U.K. daily intakes further emphasizes the need for additional research to relate intake and effect in various population groups.
采用反相高效液相色谱法测定了英国常见饮用茶中的总酚、儿茶素和咖啡因含量。茶包或茶叶购自当地超市,在沸水中提取5分钟。所得数据显示,总酚(红茶中为80.5 - 134.9毫克/克干物质(DM),绿茶中为87 - 106.2毫克/克DM)和儿茶素(红茶、绿茶和水果茶中分别为5.6 - 47.5、51.5 - 84.3和8.5 - 13.9毫克/克DM)含量存在显著差异;这很可能是由于农艺条件、叶片年龄、运输过程中和运输后的储存以及发酵程度不同所致。红茶中的咖啡因含量(22 - 28毫克/克DM)显著高于发酵程度较低的绿茶(11 - 20毫克/克DM)。五种主要茶儿茶素的相对浓度排序为表没食子儿茶素没食子酸酯(EGCG)>表儿茶素没食子酸酯(ECG)>表儿茶素(EC)>没食子儿茶素(EGC)>儿茶素(C)。根据平均每天饮用三杯茶(每杯200毫升,茶叶重量/体积比为1%)计算,英国人群从水果茶、红茶和绿茶中摄入的总茶儿茶素估计量分别为61.5、92.7和405.5毫克/天。变异系数分别为19.4%、88.6%和17.3%,表明这些摄入量存在很大差异。计算得出的咖啡因摄入量在92至146毫克/天之间。此外,许多人会饮用大量不同浓度(由冲泡条件决定)的茶。英国每日摄入量的这种广泛分布进一步强调了开展更多研究以关联不同人群摄入量与效应的必要性。