从两种不同的、相容的葡萄球菌质粒获得的重组质粒。
Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.
作者信息
Iordănescu S
出版信息
J Bacteriol. 1975 Nov;124(2):597-601. doi: 10.1128/jb.124.2.597-601.1975.
Abstract
From two different, compatible staphylococcal plasmids that determine streptomycin and chloramphenicol resistance, respectively, a recombinant plasmid was obtained. This plasmid can be transduced with a rather high frequency (10(-4)/plaque-forming unit) to plasmid-negative strains, the linkage of the two markers being 100%. The maintenance of the recombinant plasmid in the host cell seems to be controlled by the chloramphenicol resistance plasmid. The recombinant plasmid proved to be incompatible with both parental plasmids, which are unrelated. The relationship between the chloramphenicol resistance plasmid and the recombinant plasmid was the same as the between genetically marked derivatives of the recombinant plasmid, whereas the relationship of the streptomycin resistance plasmid to the recombinant plasmid was of a different, asymmetrical type.
摘要
分别从两种不同的、相容的葡萄球菌质粒（它们分别决定对链霉素和氯霉素的抗性）中获得了一种重组质粒。该质粒能够以相当高的频率（10⁻⁴/噬菌斑形成单位）转导至质粒阴性菌株，两种标记的连锁率为100%。重组质粒在宿主细胞中的维持似乎受氯霉素抗性质粒控制。已证明该重组质粒与两种不相关的亲本质粒均不相容。氯霉素抗性质粒与重组质粒之间的关系，和重组质粒的遗传标记衍生物之间的关系相同，而链霉素抗性质粒与重组质粒的关系则属于不同的、不对称类型。
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