Cen X, Wang R, Wu Z
School of Public of Health, West China University of Medical Sciences, Chengdu 610041.
Zhonghua Yu Fang Yi Xue Za Zhi. 1999 Jul;33(4):221-3.
Effect of zinc on proliferation and differentiation of osteoblast in rats was studied in vitro.
Osteoblasts were isolated from the rat calvaria and subcultured in DMEM media. Rats were divided into four groups in the study, with administration of 0 micromol/L (as control), 10 micromol/L, 25 micromol/L and 50 micromol/L of Zn(2+), respectively. DNA synthesis at varied time points was determined with incorporation of (3)H-thymidine, and cell cycle was analyzed by flow cytometry. Synthesis of collagen was measured with incorporation of (3)H-proline. Activity of alkaline phosphatase (AKP) and osteocalcin content were measured by enzyme dynamics method and radioimmunoassay, respectively.
Incorporation of (3)H-thymidine in the three groups with different zinc-supplement was significantly higher than that in the control group at each time point, and supplement with 25 micromol/L and 50 micromol/L of Zn(2+) could promote the transition of cell cycle from phases G(0)/G(1) to S phase. Syntheses of collagen and osteocalcin and activity of AKP in the groups with zinc-supplement were greater, as compared with those in the control group.
Zinc could promote proliferation and differentiation of the rat osteoblast in vitro.
在体外研究锌对大鼠成骨细胞增殖和分化的影响。
从大鼠颅骨分离成骨细胞并在DMEM培养基中传代培养。本研究将大鼠分为四组，分别给予0微摩尔/升（作为对照）、10微摩尔/升、25微摩尔/升和50微摩尔/升的锌离子。通过掺入³H-胸腺嘧啶核苷测定不同时间点的DNA合成，并通过流式细胞术分析细胞周期。通过掺入³H-脯氨酸测定胶原蛋白的合成。分别采用酶动力学法和放射免疫分析法测定碱性磷酸酶（AKP）活性和骨钙素含量。
在每个时间点，三个补充不同锌离子的组中³H-胸腺嘧啶核苷的掺入量均显著高于对照组，补充25微摩尔/升和50微摩尔/升的锌离子可促进细胞周期从G₀/G₁期向S期转变。与对照组相比，补充锌离子的组中胶原蛋白和骨钙素的合成以及AKP活性更高。
锌在体外可促进大鼠成骨细胞的增殖和分化。
