Yu Zengli, Li Wenjie, Zhang Lishi
School of Public Health, University of Zhengzhou, Zhengzhou 450052, China.
Wei Sheng Yan Jiu. 2004 Sep;33(5):569-71.
To further explore the effects of genistein (GS) on cell proliferation and differentiation in adult human osteoblast.
Human osteoblast from passage 4 were harvested and seeded in phenol red-free DMEM medium containing 5% charcoal-stripped fetal bovine serum (CS-FBS) before the addition of test compounds for 4 days. DNA synthesis was tested by 3H-thymine incorporation and cell cycle was determined by flow cytometry. Collagen protein content and alkaline phosphatase (AKP) activity were examined.
After treatment for 48 h, the presence of GS (10(-7) or 10(-6) mol/L) caused a significant increase in DNA content and accumulation at S and G2/M phage in a dose-dependent manner. Additionally, the anabolic effect of GS (10(-7) and 10(-6) mol/L) on the collage content and AKP activity were significantly enhanced in the presence of genistein (10(-7) or 10(-6) mol/L) . The effects of GS on osteoblast were completely abolished in the presence of estrogen receptor antagonist, 4-Hydroxy tamoxifen (TAM) (10(-7) mol/L).
Findings suggest that osteoblast function is promoted by genistein and that the estrogen receptors might be involved in the response, thereby playing an important role in bone remodeling. Presumably, dietary soy products are useful in the prevention of osteoporosis.
进一步探讨染料木黄酮（GS）对成人成骨细胞增殖和分化的影响。
收集第4代人成骨细胞，接种于含5%活性炭处理胎牛血清（CS-FBS）的无酚红DMEM培养基中，加入受试化合物培养4天。通过3H-胸腺嘧啶掺入法检测DNA合成，采用流式细胞术测定细胞周期。检测胶原蛋白含量和碱性磷酸酶（AKP）活性。
处理48小时后，GS（10(-7)或10(-6)mol/L）使DNA含量显著增加，并以剂量依赖方式使S期和G2/M期细胞蓄积。此外，在存在染料木黄酮（10(-7)或10(-6)mol/L）的情况下，GS（10(-7)和10(-6)mol/L）对胶原蛋白含量和AKP活性的合成代谢作用显著增强。在雌激素受体拮抗剂4-羟基他莫昔芬（TAM）（10(-7)mol/L）存在的情况下，GS对成骨细胞的作用完全被消除。
研究结果表明，染料木黄酮可促进成骨细胞功能，雌激素受体可能参与该反应，从而在骨重塑中发挥重要作用。推测膳食大豆制品对预防骨质疏松症有益。
