[c-jun在染料木黄酮对新生大鼠颅骨成骨细胞增殖和分化作用中的作用]

[Role of c-jun in the effect of genistein on neonatal rat calvaria osteoblasts proliferation and differentiation].

作者信息

Zhang Yuehong, Jin Hong, Xu Zhiqin, Nan Wenkao

机构信息

Department of Nutrition, General Hospital of PLA, Beijing 100853, China.

出版信息

Wei Sheng Yan Jiu. 2008 May;37(3):308-10.

PMID:18646529

Abstract

OBJECTIVE

To study the role of the c-jun in the effects of genistein on osteoblasts in neonatal rat calvaria cultures in vitro.

METHODS

Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the medium at the doses of 10(-5), 10(-6) and 10(-7) mol/L genistein and at the dose of 10(-9) mol/L and 10(-10) mol/L estrogen for 48h and 72h. The proliferation and DNA of osteoblasts were assayed by MTT method and H-TdR incorporation, and c-jun was measured by in situ hybridization assay.

RESULTS

Genistein significantly promoted osteoblast proliferation and DNA synthesis. The values of MTT in control and genistein and estrogen groups at 48h and 72h were 0.19, 0.15, and 0.39, 0.45, 0.46, and 0.29, 0.32, 0.37 and 0.35, 0.38, and 0.50, 0.49. The content of 3H-TdR incorporation in control and genistein and estrogen groups were 68.47, and 101, 844, 512 and 1108.8, 1204.2. There was no difference of expression of c-jun among control and genistein and estrogen groups.

CONCLUSION

Genistein could promoted osteoblast proliferation and differentiation not associated with c-jun.

摘要

目的

研究c-jun在金雀异黄素对新生大鼠颅骨成骨细胞体外作用中的作用。

方法

通过胰蛋白酶和胶原酶消化从新生大鼠颅骨分离成骨细胞，分别用10(-5)、10(-6)和10(-7)mol/L的金雀异黄素以及10(-9)mol/L和10(-10)mol/L的雌激素处理细胞48小时和72小时。采用MTT法和H-TdR掺入法检测成骨细胞的增殖和DNA情况，并用原位杂交法检测c-jun。

结果

金雀异黄素显著促进成骨细胞增殖和DNA合成。对照组、金雀异黄素组和雌激素组在48小时和72小时的MTT值分别为0.19、0.15，0.39、0.45、0.46，以及0.29、0.32、0.37和0.35、0.38、0.50、0.49。对照组、金雀异黄素组和雌激素组的3H-TdR掺入量分别为68.47、101、844、512和1108.8、1204.2。对照组、金雀异黄素组和雌激素组之间c-jun的表达无差异。