Manohar V, Talpur N A, Echard B W, Lieberman S, Preuss H G
Department of Physiology, Georgetown University Medical Center, Washington, DC 20007, USA.
Diabetes Obes Metab. 2002 Jan;4(1):43-8. doi: 10.1046/j.1463-1326.2002.00180.x.
We examined benefits of a water-soluble extract of maitake mushroom designated as Fraction X (FXM) on the glucose/insulin metabolism of insulin-resistant KK mice, and compared the results of FXM with those of a sulphonylurea, Glipizide.
In several acute studies, insulin-resistant KK mice were gavaged with a single dose of varying concentrations of FXM, or a single dose of one concentration of the oral hypoglycaemic drug, Glipizide. In the one chronic study, KK mice were gavaged with FXM, Glipizide, or an equal volume of isotonic saline (baseline control) twice daily. Retro-orbital blood was drawn on the morning of the 4th and 7th days before the early gavage. Blood glucose was measured by routine laboratory procedures, and serum insulin was estimated by a radioimmunoassay (RIA) assay developed specifically for rodents.
At a dose of FXM (140 mg/mouse), a statistically significant lowering of circulating glucose concentrations was again seen at 8-12 h and 16-18 h after oral gavage. The lowering approximated 25% of the original concentration. Oral gavage of Glipizide resulted in statistically significantly lower values of circulating glucose (25-37% lower compared with baseline) at 8-24 h post dosing. In the chronic study, the circulating concentrations of glucose and insulin of mice taking 140 mg FXM per day were decreased significantly at days 4 and 7.
FXM, a natural extract obtained from maitake mushroom, favourably influences glucose/insulin metabolism in insulin-resistant KK mice. The lowering of both circulating glucose and insulin concentrations suggests that FXM works primarily by enhancing peripheral insulin sensitivity.
我们研究了舞茸蘑菇的水溶性提取物X组分（FXM）对胰岛素抵抗的KK小鼠葡萄糖/胰岛素代谢的影响，并将FXM的结果与磺脲类药物格列吡嗪的结果进行比较。
在几项急性研究中，给胰岛素抵抗的KK小鼠灌胃单剂量不同浓度的FXM，或单剂量一种浓度的口服降糖药格列吡嗪。在一项慢性研究中，每天给KK小鼠灌胃两次FXM、格列吡嗪或等体积的等渗盐水（基线对照）。在早饲前第4天和第7天上午采集眶后血。通过常规实验室程序测量血糖，并通过专门为啮齿动物开发的放射免疫分析（RIA）测定血清胰岛素。
在FXM剂量为140mg/小鼠时，灌胃后8 - 12小时和16 - 18小时再次观察到循环葡萄糖浓度有统计学意义的降低。降低幅度约为原始浓度的25%。灌胃格列吡嗪导致给药后8 - 24小时循环葡萄糖值有统计学意义的降低（与基线相比降低25 - 37%）。在慢性研究中，每天服用140mg FXM的小鼠在第4天和第7天循环葡萄糖和胰岛素浓度显著降低。
FXM是从舞茸蘑菇中获得的天然提取物，对胰岛素抵抗的KK小鼠的葡萄糖/胰岛素代谢有有利影响。循环葡萄糖和胰岛素浓度的降低表明FXM主要通过增强外周胰岛素敏感性起作用。
