Velázquez E, Calvo O, Cervantes E, Mateos P F, Tamame M, Martínez-Molina E
Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:917-923. doi: 10.1099/00207713-50-2-917.
Staircase electrophoresis (SCE) in polyacrylamide gels was used to analyse the stable low-molecular-weight (LMW) RNA profiles of several yeast species and genera. As in prokaryotes, this new electrophoretic technique results in good separation of molecules forming LMW RNA profiles in yeasts. In this study it is reported that, while LMW RNA profiles in prokaryotes include only 5S rRNA, and class 2 and class 1 tRNA, these profiles in eukaryotes also include 5.8S rRNA. Differences in the number and distribution of RNA bands in these profiles allowed identification of differences among the yeast species and genera assayed. LMW RNAs, analysed by SCE, provide a yeast fingerprint that allows them to be clearly differentiated and will in the future enable the rapid assignment of yeast isolates to already described species and the detection of new ones.
采用聚丙烯酰胺凝胶中的阶梯式电泳（SCE）分析了几种酵母属和种的稳定低分子量（LMW）RNA图谱。与原核生物一样，这种新的电泳技术能很好地分离酵母中形成LMW RNA图谱的分子。本研究报道，原核生物中的LMW RNA图谱仅包括5S rRNA、2类和1类tRNA，而真核生物中的这些图谱还包括5.8S rRNA。这些图谱中RNA条带数量和分布的差异有助于鉴定所检测的酵母属和种之间的差异。通过SCE分析的LMW RNA提供了一种酵母指纹图谱，使其能够被清晰区分，并且在未来能够快速将酵母分离株归为已描述的物种，并检测新的物种。
