Graham D A, Calvert V, McLaren E
Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast.
Vet Rec. 2002 Feb 16;150(7):201-4. doi: 10.1136/vr.150.7.201.
Eighty-four pairs of acute and convalescent serum samples collected in 1998 and 1999 from 17 outbreaks of respiratory disease, milk drop syndrome or diarrhoea in cattle were tested by haemagglutination inhibition against human influenza viruses A/Eng/333/80 (HIN1) and A/Eng/427/88 (H3N2). Antibodies to these viruses were present in the convalescent sera of 56.5 per cent and 58.8 per cent cattle tested, respectively, with 56 per cent of the animals seroconverting to one or both viruses. Titres were typically higher to A/Eng/427/88 (H3N2). Further testing of a subset of 21 of these serum pairs against the predominant H1N1 and H3N2 human and porcine strains circulating when the samples were collected revealed that the highest reactivity, in terms of both the magnitude of the recorded titres and the number of positive sera, was to human H3N2 strains. The titres to human H1N1 strains and to both porcine subtypes were low or absent. Attempts to isolate influenza A virus from nasal mucus or swab samples from 142 cattle from 46 cases of respiratory disease and/or milk drop syndrome by passage in embryonated specific pathogen-free eggs were unsuccessful.
1998年和1999年从17起牛呼吸道疾病、乳漏综合征或腹泻疫情中采集的84对急性期和恢复期血清样本，通过血凝抑制试验检测了针对甲型流感病毒A/Eng/333/80（H1N1）和A/Eng/427/88（H3N2）的抗体。在接受检测的牛中，分别有56.5%和58.8%的恢复期血清中存在针对这些病毒的抗体，56%的动物血清转化为一种或两种病毒。针对A/Eng/427/88（H3N2）的抗体效价通常更高。对其中21对血清样本的一个子集，针对样本采集时流行的主要H1N1和H3N2人源和猪源毒株进行的进一步检测显示，就记录的抗体效价幅度和阳性血清数量而言，最高反应性是针对人源H3N2毒株。针对人源H1N1毒株以及两种猪源亚型的抗体效价很低或不存在。通过在无特定病原体的鸡胚中传代，试图从46例呼吸道疾病和/或乳漏综合征病例的142头牛的鼻黏液或拭子样本中分离甲型流感病毒，但未成功。
