Alam Saman, Liaqat Irfana, Al-Arifa Najiya, Zia Threem, Munawar Mohsin, Muzamil Ayesha
Physiology and Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan.
Immunology Laboratory, Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
Saudi J Biol Sci. 2023 Oct;30(10):103790. doi: 10.1016/j.sjbs.2023.103790. Epub 2023 Aug 23.
Obesity is a polygenic disorder which has become a global epidemic in recent years. Aim of the present study was to assess the theranostic potential of probiotics ( and local herbs (fenugreek seeds, aloe vera) on the body weight, biochemical (liver and kidney functions) and histology of some internal organs (liver, kidney, ovary, small intestine) in obese rats. In present study, nanoemulsions of probiotics and local herbs were formulated by high energy method and characterized by FTIR and UV analysis. One hundred and sixty (1 6 0) female wistar rats were divided into sixteen groups. A high-fat diet was given to induce obesity in them. Obese rats were treated with different doses of probiotics, local herbs and their combination. Weekly body weight was monitored. Rats were dissected after fifteen weeks; blood and organs were harvested for biochemical analysis and histology. Results demonstrated a protective effect of nanoemulsion of probiotics and local herbs on the central vein, glomerulus, villi and normal ovulatory function of obese rats. Rats treated with a combination of probiotics and local herbs (fenugreek seeds, aloe vera) exhibited improved levels of bilirubin (4 mg/dl to15 mg/dl), AST from (110 U/L to 18 U/L) and ALT from (52 U/L to 10U/L). Similar renoprotective effects were recorded on the overall renal function tests (RFT). A combination of probiotics and local herbs in post treatment rats improved urea (63 mg/dl to 22 mg/dl) and creatinine (0.2 mg/dl to 0.8 mg/dl) levels. It was therefore evident that a combination of probiotics and local herbs has the potential to reverse the effects of obesity on the biochemical parameters and histological architecture of liver, kidney, small intestine and ovary. The overall results indicated that probiotics have positive effects of their own, but when combined with local herbs, they produced highly effective results in obese rats and therefore can be used as a complementary option in obese individuals.
肥胖是一种多基因疾病，近年来已成为全球流行病。本研究的目的是评估益生菌和当地草药（胡芦巴籽、芦荟）对肥胖大鼠体重、生化指标（肝功能和肾功能）以及一些内脏器官（肝脏、肾脏、卵巢、小肠）组织学的治疗诊断潜力。在本研究中，通过高能法制备了益生菌和当地草药的纳米乳剂，并通过傅里叶变换红外光谱（FTIR）和紫外分析对其进行了表征。160只雌性Wistar大鼠被分为16组。给予高脂饮食以诱导它们肥胖。肥胖大鼠用不同剂量的益生菌、当地草药及其组合进行治疗。每周监测体重。15周后解剖大鼠；采集血液和器官进行生化分析和组织学检查。结果表明，益生菌和当地草药的纳米乳剂对肥胖大鼠的中央静脉、肾小球、绒毛和正常排卵功能具有保护作用。用益生菌和当地草药（胡芦巴籽、芦荟）组合治疗的大鼠胆红素水平从4mg/dl提高到15mg/dl，谷草转氨酶从110U/L降至18U/L，谷丙转氨酶从52U/L降至10U/L。在整体肾功能测试（RFT）中也记录到了类似的肾脏保护作用。治疗后大鼠中益生菌和当地草药的组合使尿素水平从63mg/dl降至22mg/dl，肌酐水平从0.2mg/dl降至0.8mg/dl。因此，很明显益生菌和当地草药的组合有可能逆转肥胖对肝脏、肾脏、小肠和卵巢生化参数和组织结构的影响。总体结果表明，益生菌自身具有积极作用，但与当地草药联合使用时，它们在肥胖大鼠中产生了高效的效果，因此可作为肥胖个体的一种补充选择。
