Nunoura Takuro, Akihara Saki, Takai Ken, Sako Yoshihiko
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Arch Microbiol. 2002 Apr;177(4):339-44. doi: 10.1007/s00203-002-0398-2. Epub 2002 Feb 7.
A novel, extremely thermophilic bacterium was isolated from a shallow marine hydrothermal vent at depth of 22 m in Tachibana Bay, Nagasaki Prefecture, Japan. Cells were gram-negative, non-spore-forming, motile rods. Growth was observed between 52 and 78 degrees C (optimum 70 degrees C), pH 5 and 8 (optimum pH 7) and 0-4.5% NaCl (optimum 1.0%). The isolate was a strictly aerobic heterotroph utilizing yeast extract and trypticase peptone. The G+C content of the genomic DNA is 69 mol%. Analysis of 16S rDNA sequences indicated that strain Ts1a is closely related to Thermaerobacter marianensis. The differences in physiology and DNA-DNA similarity between strain Ts1a and T. marianensis showed that strain Ts1a represents a new species of Thermaerobacter. The type strain of T. nagasakiensis is strain Ts1a (=JCM11223, DSM 14512).
从日本长崎县橘湾水深22米的浅海热液喷口分离出一种新型超嗜热细菌。细胞为革兰氏阴性、不形成芽孢、运动性杆状。在52至78摄氏度（最适温度70摄氏度）、pH值5至8（最适pH值7）和0至4.5%氯化钠（最适1.0%）条件下可观察到生长。该分离株是利用酵母提取物和胰蛋白酶蛋白胨的严格需氧异养菌。基因组DNA的G+C含量为69摩尔%。16S rDNA序列分析表明，Ts1a菌株与马里亚纳热杆菌密切相关。Ts1a菌株与马里亚纳热杆菌在生理和DNA-DNA相似性方面的差异表明，Ts1a菌株代表热杆菌属的一个新物种。长崎热杆菌的模式菌株为Ts1a菌株（=JCM11223，DSM 14512）。
