Cory S, Adams J M, Gerondakis S D, Miller J F, Gamble J, Wiener F, Spira J, Francke U
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
EMBO J. 1983;2(2):213-6. doi: 10.1002/j.1460-2075.1983.tb01407.x.
Murine plasmacytomas frequently exhibit a translocation of the distal region of chromosome 15 to the end of chromosome 12, where the immunoglobulin heavy chain locus resides. A candidate for the DNA across the chromosome fusion point is a cloned region of non-immunoglobulin DNA which in most plasmacytomas has recombined near the alpha heavy chain constant region gene. That the incoming DNA, provisionally designated LyR (lymphoid rearranging) DNA, does derive from chromosome 15 is shown here by blot analysis of DNA from two panels of somatic cell hybrids: hybridomas between an AKR T-lymphoma (Tikaut) and CBA mouse cells with a cytogenetically distinctive chromosome 15, and between mouse and Chinese hamster cells. LyR DNA segregated with chromosome 15 in all lines and the results assign LyR to the distal two thirds of that chromosome. This assignment, together with the previously reported high frequency of recombination between LyR and C(alpha) in plasmacytomas and associated alteration of LyR transcription suggests that translocation activates a LyR gene involved in plasmacytoma oncogenesis. Moreover, LyR rearrangement in certain T-lymphomas, such as the Tikaut line examined here, also implicate that gene in oncogenesis of some T-lymphomas.
鼠浆细胞瘤经常表现出15号染色体远端区域易位至12号染色体末端，而免疫球蛋白重链基因座位于12号染色体末端。跨越染色体融合点的DNA候选序列是一个非免疫球蛋白DNA的克隆区域，在大多数浆细胞瘤中，该区域已在α重链恒定区基因附近发生重组。通过对两组体细胞杂种的DNA进行印迹分析表明，这里临时命名为LyR（淋巴细胞重排）的导入DNA确实来自15号染色体：一组是AKR T淋巴瘤（Tikaut）与具有细胞遗传学特征性15号染色体的CBA小鼠细胞之间的杂交瘤，另一组是小鼠和中国仓鼠细胞之间的杂交瘤。在所有细胞系中，LyR DNA都与15号染色体分离，结果将LyR定位于该染色体的远端三分之二区域。这一定位，连同先前报道的浆细胞瘤中LyR与C(α)之间的高频率重组以及LyR转录的相关改变，表明易位激活了一个参与浆细胞瘤肿瘤发生的LyR基因。此外，某些T淋巴瘤（如这里检测的Tikaut细胞系）中的LyR重排也表明该基因参与了一些T淋巴瘤的肿瘤发生。
