Weidner E
Z Parasitenkd. 1975 Aug 21;47(1):1-9. doi: 10.1007/BF00418060.
Encephalitozoon cuniculi grow within ever-increasing parasitophorous vacuoles (PV) in peritoneal macrophages. The PV boundary membrane conforms to a rich arrangement of blebs; similar, but free vesicles were observed within the PV space. An iron dextran-concanavalin A marker was used to express visually clustered distributions of Con A receptors on the PV boundary blebs and free vesicles; no marker was observed on other membrane surfaces within the PV. These results, combined with the observation that the PV grows while the host cytoplasm decreases in mass, implicate the PV boundary blebs of interiorizing into vesicles by a pinocytic mechanism. Phagocytic vacuoles, secondary lysosomes and pinocytic vesicles were labeled by incubating infected macrophages in minimum essential medium with ferritin. Ferritin readily accumulated in secondary lysosomes and phagocytic vacuoles; however, ferritin was excluded from parasitophorous vacuoles containing E. cuniculi. Acid phosphatase cytochemical reaction product was observed in lysosomes and phagocytic vacuoles; however, parasitophorous vacuoles with vegetative E. cuniculi were always negative.
兔脑炎微孢子虫在腹膜巨噬细胞内不断增大的寄生泡（PV）中生长。PV的边界膜呈现出丰富的泡状结构；在PV空间内观察到了类似但游离的囊泡。使用铁葡聚糖-伴刀豆球蛋白A标记物来直观显示PV边界泡和游离囊泡上伴刀豆球蛋白A受体的聚集分布；在PV内的其他膜表面未观察到标记物。这些结果，再加上观察到PV生长时宿主细胞质质量减少，表明PV边界泡通过胞饮机制内陷形成囊泡。通过将感染的巨噬细胞在含有铁蛋白的最低必需培养基中孵育，对吞噬泡、次级溶酶体和胞饮囊泡进行了标记。铁蛋白很容易在次级溶酶体和吞噬泡中积累；然而，铁蛋白被排除在含有兔脑炎微孢子虫的寄生泡之外。在溶酶体和吞噬泡中观察到酸性磷酸酶细胞化学反应产物；然而，含有兔脑炎微孢子虫营养体的寄生泡总是呈阴性。
