Groves Jay T, Boxer Steven G
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Acc Chem Res. 2002 Mar;35(3):149-57. doi: 10.1021/ar950039m.
Phospholipid vesicles exhibit a natural tendency to fuse and assemble into a continuous single bilayer membrane on silica and several other substrate materials. The resulting supported membrane maintains many of the physical and biological characteristics of free membranes, including lateral fluidity. Recent advances, building on the supported membrane configuration, have created a wealth of opportunities for the manipulation, control, and analysis of membranes and the reaction environments they provide. The work reviewed in this Account, which can be broadly characterized as the science and technology of membrane patterning, contains three basic components: lateral diffusion control (barriers), membrane deposition techniques (microarrays), and electric field-induced lateral reorganization. Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes.
磷脂囊泡具有在二氧化硅和其他几种基底材料上融合并组装成连续单双层膜的自然倾向。由此形成的支撑膜保留了自由膜的许多物理和生物学特性，包括横向流动性。基于支撑膜结构的最新进展为膜及其所提供的反应环境的操纵、控制和分析创造了大量机会。本综述文章中所回顾的工作，大致可归类为膜图案化的科学与技术，包含三个基本组成部分：横向扩散控制（屏障）、膜沉积技术（微阵列）以及电场诱导的横向重组。总体而言，这些制备性和分析性的图案化膜技术为脂质膜的研究和利用提供了一个广阔的实验平台。
