Guillot J, Demanche C, Hugot J P, Berthelemy M, Wakefield A E, Dei-Cas E, Chermette R
UMR INRA-AFSSA-ENVA-Paris XII Biologie Moléculaire et Immunologie Parasitaires et Fongiques, Ecole Nationale Vétérinaire d'Alfort, Maisons Alfort, France.
J Eukaryot Microbiol. 2001;Suppl:113S-115S. doi: 10.1111/j.1550-7408.2001.tb00475.x.
The single name Pneumocystis carinii consists of an heterogeneous group of specific fungal organisms that colonize a very wide range of mammalian hosts. In the present study, mitochondrial large subunit (mtLSU) and small subunit (mtSSU) rRNA sequences of P. carinii organisms from 24 different mammalian species were compared. The mammals were included in six major groups: Primates (12 species), Rodents (5 species), Carnivores (3 species), Bats (1 species), Lagomorphs (1 species), Marsupials (1 species) and Ungulates (1 species). Direct sequencing of PCR products demonstrated that specific mtSSU and mtLSU rRNA Pneumocystis sequence could be attributed to each mammalian species. No animal harbored P. carinii f. sp. hominis. Comparison of combined mtLSU and mtSSU aligned sequences confirmed cospeciation of P. carinii and corresponding mammalian hosts. P. carinii organisms isolated from mammals of the same zoological group systematically clustered together. Within each cluster, the genetic divergence between P. carinii organisms varied in terms of the phylogenetic divergence existing among the corresponding host species. However, the relative position of P. carinii groups (rodent, camivore or primate-derived P. carinii) could not be clearly determined. Further resolution will require the integration of additional sequence data.
卡氏肺孢子虫这一名称涵盖了一组异质性的特定真菌生物，它们可寄生于范围广泛的哺乳动物宿主。在本研究中，对来自24种不同哺乳动物物种的卡氏肺孢子虫的线粒体大亚基（mtLSU）和小亚基（mtSSU）rRNA序列进行了比较。这些哺乳动物分为六大类：灵长目（12种）、啮齿目（5种）、食肉目（3种）、蝙蝠（1种）、兔形目（1种）、有袋目（1种）和偶蹄目（1种）。PCR产物的直接测序表明，特定的卡氏肺孢子虫mtSSU和mtLSU rRNA序列可归因于每个哺乳动物物种。没有动物携带人型卡氏肺孢子虫。对合并的mtLSU和mtSSU比对序列的比较证实了卡氏肺孢子虫与相应哺乳动物宿主的共同物种形成。从同一动物学类群的哺乳动物中分离出的卡氏肺孢子虫生物系统地聚集在一起。在每个聚类中，卡氏肺孢子虫生物之间的遗传差异因相应宿主物种之间存在的系统发育差异而有所不同。然而，卡氏肺孢子虫类群（啮齿动物、食肉动物或灵长类动物衍生的卡氏肺孢子虫）的相对位置无法明确确定。进一步的解析将需要整合更多的序列数据。
