Williams C D, Markov M S, Hardman W E, Cameron I L
EMF Therapeutics, Inc., Chattanooga, TN 37405, USA.
Anticancer Res. 2001 Nov-Dec;21(6A):3887-91.
A new approach to cancer therapy based on the application of therapeutic electromagnetic fields (TEMF) has been developed by EMF Therapeutics, Inc., Chattanooga, TN, USA. This study was designed to assess the effect of TEMF on tumor vascularization and growth of murine 16/C mammary adenocarcinoma cells in C3H/HeJ mice.
Implanted tumors were allowed to grow for seven days until the tumor volume reached 100 mm3 before treatment was started. Mice (20 per control, 10 per EMF exposed group) received treatment (10 minutes per day with 0, 10 mT, 15 mT or 20 mT) with a 120 pulses per second pulsating magnetic field. Tumor growth was assessed throughout the treatment period. The extent of tumor vascularization was evaluated by immunohistochemical staining for CD31.
Exposure to TEMF significantly reduced tumor growth, significantly reduced the percentage of area stained for CD31 indicating a reduction in the extent of vascularization and there was a concomitant increase in the extent of tumor necrosis.
A novel TEMF treatment safely reduced growth and vascularization of implanted breast cancers in mice.
TEMF may prove a useful adjuvant to increase the therapeutic index of conventional cancer therapy.
美国田纳西州查塔努加市的电磁疗法公司（EMF Therapeutics, Inc.）研发了一种基于治疗性电磁场（TEMF）应用的癌症治疗新方法。本研究旨在评估TEMF对C3H/HeJ小鼠体内鼠源16/C乳腺腺癌细胞的肿瘤血管生成及生长的影响。
植入的肿瘤生长7天，直至肿瘤体积达到100立方毫米后开始治疗。小鼠（对照组每组20只，电磁场暴露组每组10只）接受每秒120次脉冲的脉动磁场治疗（每天10分钟，磁场强度分别为0、10毫特斯拉、15毫特斯拉或20毫特斯拉）。在整个治疗期间评估肿瘤生长情况。通过对CD31进行免疫组织化学染色来评估肿瘤血管生成程度。
暴露于TEMF可显著降低肿瘤生长，显著降低CD31染色面积百分比，表明血管生成程度降低，同时肿瘤坏死程度增加。
一种新型TEMF治疗可安全地降低小鼠体内植入性乳腺癌的生长及血管生成。
TEMF可能被证明是一种有用的辅助手段，可提高传统癌症治疗的治疗指数。
