Kim Yong, Kim Song-Bae, You Young-Jae, Ahn Byung-Zun
Planta Med. 2002 Mar;68(3):271-4. doi: 10.1055/s-2002-23140.
The petroleum ether fraction of Pulsatilla koreana (Ranunculaceae) was found to have an inhibitory effect on the tube-like formation of human umbilical venous endothelial (HUVE) cells and strong cytotoxic activity against five tumor cell lines. The active component isolated from the fraction was deoxypodophyllotoxin (DPT). The cytotoxic activity against the tumor cells comprising the A549, SK-OV-3, SK-MEL-2, HCT15, and B16F10 cell lines, expressed as ED50, ranged from 6 to 18 ng/ml. 3 ng/ml of DPT, a concentration considerably below the cytotoxic concentration, completely inhibited the tube-like formation of HUVE cells. Furthermore, DPT exhibited an inhibition ratio of 60 % on BDF1 mice bearing Lewis lung carcinoma cells. The inhibitory effect on the tube-like formation was suggested to play an important role in antitumor activity.
白头翁（毛茛科）的石油醚提取物对人脐静脉内皮（HUVE）细胞的管状形成具有抑制作用，并且对五种肿瘤细胞系具有较强的细胞毒活性。从该提取物中分离出的活性成分是脱氧鬼臼毒素（DPT）。对A549、SK-OV-3、SK-MEL-2、HCT15和B16F10细胞系等肿瘤细胞的细胞毒活性，以半数有效剂量（ED50）表示，范围为6至18 ng/ml。3 ng/ml的DPT，这一浓度远低于细胞毒浓度，却能完全抑制HUVE细胞的管状形成。此外，DPT对携带Lewis肺癌细胞的BDF1小鼠的抑制率为60%。对管状形成的抑制作用被认为在抗肿瘤活性中起重要作用。
