Fadhel Zaineb A, Amran Suham
College of Pharmacy, University of Petra, P.O. Box 961343, Amman, Jordan.
Phytother Res. 2002 Mar;16 Suppl 1:S28-32. doi: 10.1002/ptr.793.
Previous studies have shown that green tea and black tea have antioxidant effects and chemopreventive activity against chronic disease including some forms of cancer. We have, therefore, examined the effects of an aqueous extract of black tea against carbon tetrachloride-induced lipid peroxidation as determined by the formation of thiobarbituric acid reactive substances in liver, kidneys and testes of rats. A 0.7% black tea extract was used which contained 2 mg of black tea extract solids per mL. Black tea was administered as drinking water for 3, 6, 9 and 12 months before and during carbon tetrachloride (CCl(4)) treatment in female and male rats. Rats were treated with a single oral dose of CCl(4) 1.0 mL/kg. All rats were killed 24 h after CCl(4) treatment. All animals were dosed with CCl(4) at the end of the 3, 6, 9, and 12 month of treatment. Black tea treatment for 75 days produced a decrease in CCl(4)-induced hepatic lipid peroxidation but significant decreases in thiobarbituric acid reactive substances occurred 3 months after treatment in both female and male rats. In liver and kidneys, black tea alone increased lipid peroxidation by 30%-50% in female and male rats. However, black tea decreased CCl(4)-induced lipid peroxidation in liver of female and male rats by approximately 49% and 37%, respectively. Black tea decreased CCl(4)-induced lipid peroxidation in testes by approximately 37% at a dose of 1.0 mL CCl(4)/kg. These results suggest that the protective effects of black tea against CCl(4)-induced lipid peroxidation in liver, kidneys and testes is due at least partly to its antioxidant properties, scavenging CCl(4)-associated free radicals.
先前的研究表明，绿茶和红茶具有抗氧化作用以及针对包括某些癌症形式在内的慢性疾病的化学预防活性。因此，我们检测了红茶水提取物对四氯化碳诱导的脂质过氧化的影响，该影响通过大鼠肝脏、肾脏和睾丸中硫代巴比妥酸反应性物质的形成来确定。使用了0.7%的红茶提取物，每毫升含有2毫克红茶提取物固体。在雌性和雄性大鼠接受四氯化碳（CCl₄）处理之前和期间，将红茶作为饮用水给予3、6、9和12个月。大鼠经口单次给予1.0 mL/kg的CCl₄。在CCl₄处理后24小时处死所有大鼠。在处理的第3、6、9和12个月末给所有动物注射CCl₄。红茶处理75天可使CCl₄诱导的肝脏脂质过氧化减少，但在雌性和雄性大鼠中，处理3个月后硫代巴比妥酸反应性物质显著减少。在肝脏和肾脏中，单独的红茶使雌性和雄性大鼠的脂质过氧化增加30% - 50%。然而，红茶使雌性和雄性大鼠肝脏中CCl₄诱导的脂质过氧化分别降低约49%和37%。在给予1.0 mL CCl₄/kg剂量时，红茶使睾丸中CCl₄诱导的脂质过氧化降低约37%。这些结果表明，红茶对CCl₄诱导的肝脏、肾脏和睾丸脂质过氧化的保护作用至少部分归因于其抗氧化特性，即清除与CCl₄相关的自由基。
