Sano M, Takahashi Y, Yoshino K, Shimoi K, Nakamura Y, Tomita I, Oguni I, Konomoto H
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Biol Pharm Bull. 1995 Jul;18(7):1006-8. doi: 10.1248/bpb.18.1006.
The antioxidant effects in the liver and kidney obtained from rat fed diets containing 3% green or black tea leaf powder, which were prepared from the same lot tea leaves, were studied using the tissue slice-antioxidant evaluation method with two lipid peroxidation inducers. After 50 d on the diets, liver slices prepared from green and black tea-supplemented rats showed significant inhibitory effects against tert-butyl hydroperoxide-induced lipid peroxidation. These effects, however, were not proportional to the amounts of (-)-epicatechins and antioxidant vitamins in the tea leaves. In the kidney, the antioxidant effect was observed only in the green tea-fed group. A similar antioxidant effect on the kidney was observed after oral administration of a major tea polyphenol, (-)-epigallocatechin gallate (50 mg/kg body weight for 7 d). Liver slices from black tea-fed rats also inhibited bromotrichloromethane-induced lipid peroxidation. These results demonstrated that dietary green and black tea had antioxidant effects on tissue lipid peroxidation ex vivo.
采用组织切片 - 抗氧化评估方法，使用两种脂质过氧化诱导剂，研究了由同一批次茶叶制备的含3%绿茶或红茶叶粉的饲料喂养大鼠后，其肝脏和肾脏中的抗氧化作用。在喂食饲料50天后，从补充绿茶和红茶的大鼠制备的肝脏切片对叔丁基过氧化氢诱导的脂质过氧化显示出显著的抑制作用。然而，这些作用与茶叶中（-）-表儿茶素和抗氧化维生素的含量不成正比。在肾脏中，仅在喂食绿茶的组中观察到抗氧化作用。口服主要的茶多酚（-）-表没食子儿茶素没食子酸酯（50毫克/千克体重，持续7天）后，在肾脏中观察到了类似的抗氧化作用。喂食红茶的大鼠的肝脏切片也抑制了溴三氯甲烷诱导的脂质过氧化。这些结果表明，饮食中的绿茶和红茶对离体组织脂质过氧化具有抗氧化作用。
