Psotová Jitka, Chlopcíková Sárka, Grambal Frantisek, Simánek Vilím, Ulrichová Jitka
Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacký University, Hnĕvotínská 3, 775 15 Olomouc, Czech Republic.
Phytother Res. 2002 Mar;16 Suppl 1:S63-7. doi: 10.1002/ptr.811.
Silymarin, an extract of Silybum marianum seeds, and the constituent flavonolignans silybin, silydianin and silychristin, as well as the flavonol quercetin, protected rat heart microsomes and mitochondria against iron-dependent doxorubicin induced lipid peroxidation. Quercetin was found to be more potent than either silymarin or its three constituents, whose cytoprotectivity was comparable. The radical scavenging activity of the compounds was investigated using a DPPH colour reduction assay and cyclic voltametry to assess their antioxidant activities. In contrast to quercetin, silybin, silydianin and silychristin did not chelate iron in aqueous solution. The results suggest that silymarin may prevent doxorubicin-mediated damage to rat heart membrane primarily through a free radical scavenging mechanism.
水飞蓟素，一种水飞蓟种子的提取物，以及其成分黄酮木脂素水飞蓟宾、水飞蓟宁和水飞蓟亭，还有黄酮醇槲皮素，可保护大鼠心脏微粒体和线粒体免受铁依赖性阿霉素诱导的脂质过氧化作用。研究发现，槲皮素的保护作用比水飞蓟素或其三种成分更强，而后三者的细胞保护活性相当。通过DPPH颜色还原试验和循环伏安法研究了这些化合物的自由基清除活性，以评估其抗氧化活性。与槲皮素不同，水飞蓟宾、水飞蓟宁和水飞蓟亭在水溶液中不螯合铁。结果表明，水飞蓟素可能主要通过自由基清除机制预防阿霉素介导的大鼠心脏膜损伤。
