Eigelsbach H T, Hunter D H, Janssen W A, Dangerfield H G, Rabinowitz S G
Infect Immun. 1975 Nov;12(5):999-1005. doi: 10.1128/iai.12.5.999-1005.1975.
To assess cell-mediated immunity in terms of host protection, an experimental model was developed in which passively transferred spleen cells from immunized AKR/J mice enabled nonimmume syngeneic recipients to survive an otherwise fatal infection with fully virulent Francisella tularensis. Donor immunization was achieved by administering live attenuted tularemia vaccine and, subsequently, the virulent streptomycin-sensitive SCHU S4 strain of F. tularensis. At selected intervals after immunization, donor spleen cells were transferred to streptomycin-treated recipients challenged subcutaneously, intravenously, or intraperitoneally with 25 to 50 minimal lethal doses of virulent streptomycin-resistant F. tularensis SCHU S5. The protection afforded by immune spleen cells was maximal (essentially 100%) 12 days after the SCHU S4 secondary immunization.
为了从宿主保护的角度评估细胞介导的免疫，建立了一个实验模型，在该模型中，来自免疫的AKR/J小鼠的被动转移脾细胞能使同基因非免疫受体在感染完全毒力的土拉弗朗西斯菌后存活下来，否则这种感染是致命的。通过接种减毒活兔热病疫苗，随后接种毒力强的链霉素敏感的土拉弗朗西斯菌SCHU S4菌株来实现供体免疫。在免疫后的选定时间间隔，将供体脾细胞转移到经链霉素处理的受体，这些受体通过皮下、静脉或腹腔注射25至50个最小致死剂量的毒力强的链霉素耐药土拉弗朗西斯菌SCHU S5进行攻击。在SCHU S4二次免疫后12天，免疫脾细胞提供的保护作用最大（基本上为100%）。
