Kojima Takashi, Uesugi Takehiko, Toda Toshiya, Miura Yutaka, Yagasaki Kazumi
Department of Applied Biological Science, Tokyo Noko University, Fuchu, Japan.
Lipids. 2002 Mar;37(3):261-5. doi: 10.1007/s11745-002-0889-z.
Effects of genistein and its glycoside genistin were studied in nephritic rats with endogenous hyperlipidemia. Male Wistar rats with glomerulonephritis caused by a single intravenous injection of nephrotoxic serum were orally given 5 mg of genistein or 8 mg of genistin/d/100 g body weight for 12 d. These isoflavones suppressed nephritis-induced severe hypercholesterolemia and hypertriglyceridemia, and their hypolipidemic action was almost identical. Fecal steroid excretion was unchanged by administration of the two isoflavones. Genistein inhibited the incorporation of [1-14C]acetate into cholesterol and FA in liver slices from nephritic rats when added to an incubation buffer, whereas genistin did not. These results suggest that genistin may be hydrolyzed to genistein and that genistein itself and/or its metabolite(s) may be intracorporal entities suppressing hepatic lipid syntheses. They also suggest that the suppression of hepatic lipid synthesis may be one mechanism of the hypolipidemic action of genistein.
研究了染料木黄酮及其糖苷染料木苷对患有内源性高脂血症的肾炎大鼠的影响。通过单次静脉注射肾毒性血清诱导肾小球肾炎的雄性Wistar大鼠，以5毫克染料木黄酮或8毫克染料木苷/天/100克体重的剂量口服给药12天。这些异黄酮抑制了肾炎诱导的严重高胆固醇血症和高甘油三酯血症，它们的降血脂作用几乎相同。给予这两种异黄酮后，粪便类固醇排泄没有变化。当添加到孵育缓冲液中时，染料木黄酮抑制了[1-¹⁴C]乙酸盐掺入肾炎大鼠肝脏切片中的胆固醇和脂肪酸中，而染料木苷则没有。这些结果表明，染料木苷可能被水解为染料木黄酮，并且染料木黄酮本身和/或其代谢产物可能是体内抑制肝脏脂质合成的物质。它们还表明，抑制肝脏脂质合成可能是染料木黄酮降血脂作用的一种机制。
