伊朗设拉子输血研究所供血中抗弓形虫抗体水平

Anti-Toxoplasma gondii Antibody Levels in Blood Supply of Shiraz Blood Transfusion Institute, Iran.

作者信息

Shaddel Minoo, Mirzaii-Dizgah Iraj, Hoshangi Mehran

机构信息

Dept. of Parasitology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Dept. of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Parasitol. 2014 Mar;9(1):120-4.

PMID:25642268

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4289869/

Abstract

BACKGROUND

The prevalence of Toxoplasma gondii infection in the blood donors has been poorly studied. The aim of this study was to assess the prevalence of acute and chronic toxoplasmosis in blood products.

METHODS

A total of 250 blood products (112 fresh frozen plasma and 138 packed cells) in the Blood Transfusion Institute, Shiraz, Iran were tested for specific T. gondii antibodies (IgG and IgM) by ELISA method in 2013. Positive IgG anti-T. gondii samples were further tested for IgM anti-T. gondii antibody. A positive IgG test with the negative and positive IgM test was interpreted as a chronic and acute toxoplasmosis, respectively. The relationship of jobs, blood types, sex, marital status and residency of participants with chronic toxoplasmosis prevalence were statistically analyzed by χ(2).

RESULTS

Of 250 samples, 58 (23.2%) and one were positive for IgG anti-T. T. gondii (chronic) and IgM anti-T. T. gondii (acute) antibodies levels, respectively. Twenty nine (25.9%) of fresh frozen plasma (FFP) samples were positive for IgG anti-T. gondiiiand 1(0.89%) of them was positive for IgM anti-T. gondiii antibody. Thirty (21.74%) of packed cell samples were positive for IgG anti-T. gondii antibody. The prevalence of chronic toxoplasmosis was significantly higher in workers, farmers, house wives, unemployed and free jobs (P=0.007), people with low education levels (P=0.035) and B type of blood ABO system (P=0.0001). However, there were no significant differences regarding to age, sex, marital status, residency and type of blood products.

CONCLUSIONS

There were chronic and acute toxoplasmosis in blood products and the prevalence of toxoplasmosis especially chronic form was high. Therefore screening of blood for T. gondii antibodies may be considered.

摘要

背景

献血者中弓形虫感染的患病率研究较少。本研究旨在评估血液制品中急性和慢性弓形虫病的患病率。

方法

2013年，采用酶联免疫吸附测定法（ELISA）对伊朗设拉子输血研究所的250份血液制品（112份新鲜冰冻血浆和138份红细胞悬液）进行了特异性弓形虫抗体（IgG和IgM）检测。对IgG抗弓形虫阳性样本进一步检测IgM抗弓形虫抗体。IgG检测阳性且IgM检测阴性和阳性分别被解释为慢性和急性弓形虫病。采用χ²检验对参与者的工作、血型、性别、婚姻状况和居住地与慢性弓形虫病患病率的关系进行统计学分析。

结果

在250份样本中，IgG抗弓形虫（慢性）和IgM抗弓形虫（急性）抗体水平分别有58份（23.2%）和1份呈阳性。29份（25.9%）新鲜冰冻血浆（FFP）样本IgG抗弓形虫呈阳性，其中1份（0.89%）IgM抗弓形虫抗体呈阳性。30份（21.74%）红细胞悬液样本IgG抗弓形虫抗体呈阳性。慢性弓形虫病的患病率在工人、农民、家庭主妇、失业者和自由职业者中显著较高（P = 0.007），在低教育水平人群中较高（P = 0.035），在ABO血型系统的B型血人群中较高（P = 0.0001）。然而，在年龄、性别、婚姻状况、居住地和血液制品类型方面没有显著差异。