Andersson Per, Edman Kjell, Lindberg A M
Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82, Sweden.
Virus Res. 2002 Apr 23;85(1):71-83. doi: 10.1016/s0168-1702(02)00019-9.
Echovirus 18 (EV18) is one of the echovirus serotypes associated with human diseases and in particular aseptic meningitis. To facilitate studies of the molecular epidemiology of EV18 and the evolution of enteroviruses in general, the complete nucleotide (nt) sequence was determined for the echovirus 18 prototype strain (Metcalf, EV18M). Excluding the poly A sequence, the genome consists of 7410 nt divided into a 740 nt 5' untranslated region (5' UTR), a 6567 nt long open reading frame coding for a 2189 amino acid (aa) polyprotein and a 103 nt 3' UTR. Molecular analysis of the EV18M genome showed a typical enterovirus-like organization. Phylogenetic analysis of the structural and non-structural genes revealed a pattern of different relationships to other echo- and coxsackieviruses. Similarity analysis demonstrated that the Hill strain of echovirus 9 is most likely the result of a previous recombination event between ancestors of the echovirus 9 strain Barty (5' half of the genome) and EV18M (3' half). Using a maximum likelihood approach, the recombination point was mapped to the 2C gene.
肠道病毒18型（EV18）是与人类疾病尤其是无菌性脑膜炎相关的肠道病毒血清型之一。为了促进对EV18分子流行病学及一般肠道病毒进化的研究，测定了肠道病毒18型原型株（梅特卡夫，EV18M）的完整核苷酸（nt）序列。除去多聚腺苷酸序列，基因组由7410个核苷酸组成，分为一个740 nt的5'非翻译区（5'UTR）、一个6567 nt长的开放阅读框，编码一个2189个氨基酸（aa）的多聚蛋白以及一个103 nt的3'UTR。对EV18M基因组的分子分析显示出典型的肠道病毒样结构。对结构基因和非结构基因的系统发育分析揭示了与其他埃可病毒和柯萨奇病毒的不同关系模式。相似性分析表明，肠道病毒9型希尔株很可能是先前肠道病毒9型巴蒂株（基因组的5'一半）和EV18M（3'一半）的祖先之间发生重组事件的结果。使用最大似然法，将重组点定位到2C基因。
