Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Virology Laboratory, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
WHO WPRO Regional Polio Reference Laboratory and Key Laboratory of Medical Virology and Viral Diseases, National Health and Family Planning Commission of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Virol Sin. 2019 Feb;34(1):50-58. doi: 10.1007/s12250-018-0080-8. Epub 2019 Feb 21.
Echovirus 18 (E18), a serotype of Enterovirus B (EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in mainland China, but became the predominant pathogen associated with viral encephalitis (VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in mainland China, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease (HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from mainland China after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 5'-untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiple-recombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics.
肠道病毒 18 型（E18）是肠道病毒 B （EV-B）种的一个血清型，是无菌性脑膜炎的重要病原体。E18 在中国大陆很少被检测到，但在 2015 年首次成为河北省病毒性脑炎（VE）和脑膜炎的主要病原体。为了研究 E18 在中国大陆的分子流行病学和遗传特征，从 2015 年至 2016 年期间，从中国六个省份的手足口病（HFMD）患者咽喉拭子中获得了 16 株 E18 株，从河北省 2015 年 18 例 VE 患者的脑脊液标本中获得了 4 株 E18 株，并进行了测序。结合 GenBank 数据库中的序列，我们进行了广泛的遗传分析。VP1 基因序列的系统进化分析显示，2015 年后中国大陆的所有 E18 株均属于亚基因组 C2。HFMD 和 VE/脑膜炎株的 E18 基因组序列在系统进化和变异分析方面没有明显的特异性差异。中国 E18 株在 5'-非翻译区和 P2 和 P3 非结构蛋白编码区可能发生了潜在的多次重组。目前的 E18 株是潜在的多重组病毒。总体而言，这些发现表明 E18 引起了 HFMD、VE 和脑膜炎，尽管临床特征与病毒基因组特征之间没有明显的关联。
