Weir Susan C, Pokorny Nicholas J, Carreno Ramon A, Trevors Jack T, Lee Hung
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Appl Environ Microbiol. 2002 May;68(5):2576-9. doi: 10.1128/AEM.68.5.2576-2579.2002.
Nine liquid disinfectants were tested for their ability to reduce infectivity of Cryptosporidium parvum oocysts in cell culture. A 4-min exposure to 6% hydrogen peroxide and a 13-min exposure to ammonium hydroxide-amended windshield washer fluid reduced infectivity 1,000-fold. Other disinfectants tested (70% ethanol, 37% methanol, 6% sodium hypochlorite, 70% isopropanol, and three commercial disinfectants) did not reduce the infectivity after a 33-min exposure. The results indicate that hydrogen peroxide and windshield washer fluid or ammonium hydroxide disinfectant may be suitable laboratory disinfectants against C. parvum oocysts.
对九种液体消毒剂进行了测试,以评估它们在细胞培养中降低微小隐孢子虫卵囊感染性的能力。将微小隐孢子虫卵囊暴露于6%的过氧化氢4分钟以及暴露于添加了氢氧化铵的挡风玻璃清洗液13分钟,可使感染性降低1000倍。所测试的其他消毒剂(70%乙醇、37%甲醇、6%次氯酸钠、70%异丙醇以及三种商用消毒剂)在暴露33分钟后并未降低感染性。结果表明,过氧化氢以及挡风玻璃清洗液或氢氧化铵消毒剂可能是适用于针对微小隐孢子虫卵囊的实验室消毒剂。