Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany,
Parasitol Res. 2014 Aug;113(8):2983-9. doi: 10.1007/s00436-014-3960-3. Epub 2014 Jun 1.
The aim of this study was to determine the effect of different concentrations of table salt (NaCl) and ethanol (v/v) solutions on the viability of Alaria alata mesocercariae. Furthermore, the survival of A. alata mesocercariae during simulated human gastric digestion was evaluated. For this purpose, A. alata mesocercariae migration technique (AMT) was used for the isolation of the parasite from high-positive A. alata mesocercariae meat from wild boar, raccoon, raccoon dog, and badger meat. In total, we have studied the behavior of 582 larvae under different conditions (NaCl, ethanol, and artificial gastric juice) in three independent in vitro experiments. The larvae survived at a NaCl concentration of up to 2.0% until day 21 with a median survival time of 11 days. At 3.0% NaCl concentration, the larvae lost their vitality after less than 24 h. In addition, it was found that ethanol concentrations from 8.0 to 70.0% were effective at reducing survival of A. alata mesocercariae within a short period of time (<1 min). Finally, our studies have revealed that it required 120 min to reliably inactivate all A. alata mesocercariae within HCl-pepsin digestion solution with a pH of 1.5-2.0 at 37°C. Consequently, the results showed that 3.0% is the minimum concentration of NaCl in meat products recommended for human consumption because at lower NaCl concentration the parasite survived for a substantial period of time. Finally, the common concentrations of ethanol used for the disinfection of surfaces in household and/or laboratory, are sufficient for the inactivation of A. alata mesocercariae.
本研究旨在确定不同浓度的食盐（NaCl）和乙醇（v/v）溶液对海兽胃线虫中绦期幼虫活力的影响。此外，还评估了海兽胃线虫中绦期幼虫在模拟人类胃消化过程中的存活情况。为此，采用海兽胃线虫迁移技术（AMT）从野猪、浣熊、貉和獾肉中高阳性海兽胃线虫中绦期幼虫肉中分离寄生虫。总共，我们在三个独立的体外实验中研究了 582 条幼虫在不同条件（NaCl、乙醇和人工胃液）下的行为。幼虫在 2.0%的 NaCl 浓度下可存活至第 21 天，中位存活时间为 11 天。在 3.0%的 NaCl 浓度下，幼虫在不到 24 小时内失去活力。此外，发现乙醇浓度在 8.0%至 70.0%之间可在短时间内（<1 分钟）有效降低海兽胃线虫中绦期幼虫的存活率。最后，我们的研究表明，在 pH 值为 1.5-2.0、37°C 的 HCl-胃蛋白酶消化溶液中，需要 120 分钟才能可靠地使所有海兽胃线虫中绦期幼虫失活。因此，结果表明，3.0%是推荐人类食用的肉制品中 NaCl 的最小浓度，因为在较低的 NaCl 浓度下，寄生虫会存活很长一段时间。最后，家庭和/或实验室中用于表面消毒的常见乙醇浓度足以使海兽胃线虫中绦期幼虫失活。
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