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表达沃尔巴克氏体蛋白的嵌合体共生体可刺激蚊子的免疫反应并抑制丝虫寄生虫的发育。

Chimeric symbionts expressing a Wolbachia protein stimulate mosquito immunity and inhibit filarial parasite development.

机构信息

Department of Biosciences and Pediatric Clinical Research Center "Romeo and Enrica Invernizzi", University of Milan, Milan, Italy.

Centro Interuniversitario di Ricerca sulla Malaria/Italian Malaria Network, Milan, Italy.

出版信息

Commun Biol. 2020 Mar 6;3(1):105. doi: 10.1038/s42003-020-0835-2.

DOI:10.1038/s42003-020-0835-2
PMID:32144396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7060271/
Abstract

Wolbachia can reduce the capability of mosquitoes to transmit infectious diseases to humans and is currently exploited in campaigns for the control of arboviruses, like dengue and Zika. Under the assumption that Wolbachia-mediated activation of insect immunity plays a role in the reduction of mosquito vectorial capacity, we focused our attention on the Wolbachia surface protein (WSP), a potential inductor of innate immunity. We hypothesized that the heterologous expression of this protein in gut- and tissue-associated symbionts may reduce parasite transmission. We thus engineered the mosquito bacterial symbiont Asaia to express WSP (Asaia). Asaia induced activation of the host immune response in Aedes aegypti and Anopheles stephensi mosquitoes, and inhibited the development of the heartworm parasite Dirofilaria immitis in Ae. aegypti. These results consolidate previous evidence on the immune-stimulating property of WSP and make Asaia worth of further investigations as a potential tool for the control of mosquito-borne diseases.

摘要

沃尔巴克氏体可以降低蚊子将传染病传播给人类的能力，目前被用于控制登革热和寨卡等虫媒病毒的活动中。鉴于沃尔巴克氏体介导的昆虫免疫激活在降低蚊子媒介能力方面发挥作用，我们将注意力集中在沃尔巴克氏体表面蛋白（WSP）上，这是一种潜在的先天免疫诱导剂。我们假设该蛋白在肠道和组织相关共生体中的异源表达可能会降低寄生虫的传播。因此，我们设计了蚊子共生菌亚洲菌来表达 WSP（Asaia）。亚洲菌诱导埃及伊蚊和致倦库蚊的宿主免疫反应激活，并抑制埃及伊蚊中心丝虫寄生虫的发育。这些结果巩固了之前关于 WSP 免疫刺激特性的证据，并使亚洲菌成为一种有潜力的控制蚊媒疾病的工具，值得进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/4519957b6c07/42003_2020_835_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/092176f6e7fe/42003_2020_835_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/fedf6697bf4d/42003_2020_835_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/b79416528f68/42003_2020_835_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/43d8220c3832/42003_2020_835_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/4519957b6c07/42003_2020_835_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/092176f6e7fe/42003_2020_835_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/fedf6697bf4d/42003_2020_835_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/b79416528f68/42003_2020_835_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/43d8220c3832/42003_2020_835_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38c/7060271/4519957b6c07/42003_2020_835_Fig5_HTML.jpg

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First report of natural Wolbachia infection in the malaria mosquito Anopheles arabiensis in Tanzania.坦桑尼亚自然感染沃尔巴克氏体的疟蚊按蚊属的首次报告。
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Field assessment of potential sugar feeding stations for disseminating bacteria in a paratransgenic approach to control malaria.
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Wolbachia infection in Aedes aegypti does not affect its vectorial capacity for Dirofilaria immitis.沃尔巴克氏体感染埃及伊蚊不影响其对犬心丝虫的传播能力。
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