Condon Ciarán, Rourera Jordi, Brechemier-Baey Dominique, Putzer Harald
UPR 9073, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France.
J Bacteriol. 2002 May;184(10):2845-9. doi: 10.1128/JB.184.10.2845-2849.2002.
In Bacillus subtilis, maturation of 5S rRNA is catalyzed by an enzyme called RNase M5. We searched for potential mRNA substrates for RNase M5 by gene array technology, based on the premise that most endonucleolytic cleavages have an effect on the stability of RNA and hence on steady-state levels of expression. Only a handful of genes had significantly altered expression in rnmV mutants compared to wild-type strains that could subsequently be confirmed by Northern blotting. The effect of RNase M5 on the expression of the best candidates, the odhAB and sucCD operons, is indirect, by a mechanism we do not yet understand. We show that an effect of RNase M5 on the expression of the remaining candidate, ctsR, is due to the failure to process the 5S rRNA contained in the rrnW lying directly upstream. We thus conclude that RNase M5 has very few or possibly no mRNA substrates in B. subtilis.
在枯草芽孢杆菌中，5S rRNA的成熟由一种名为RNase M5的酶催化。基于大多数核酸内切酶切割会影响RNA稳定性并进而影响稳态表达水平这一前提，我们通过基因阵列技术寻找RNase M5的潜在mRNA底物。与野生型菌株相比，在rnmV突变体中只有少数基因的表达发生了显著变化，随后可通过Northern印迹法予以证实。RNase M5对最佳候选基因odhAB和sucCD操纵子表达的影响是间接的，其机制我们尚不清楚。我们发现，RNase M5对其余候选基因ctsR表达的影响是由于未能加工直接位于上游的rrnW中所含的5S rRNA。因此，我们得出结论，RNase M5在枯草芽孢杆菌中几乎没有或可能没有mRNA底物。
