Shu Pei-Yun, Chen Li-Kuang, Chang Shu-Fen, Yueh Yi-Yun, Chow Ling, Chien Li-Jung, Chin Chuan, Yang Hui-Hua, Lin Ting-Hsiang, Huang Jyh-Hsiung
Division of Research and Diagnosis, Center for Disease Control, Department of Health, 161 Kun-Wang Street, Taipei, Taiwan, Republic of China.
J Clin Microbiol. 2002 May;40(5):1840-4. doi: 10.1128/JCM.40.5.1840-1844.2002.
An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents of Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during 1997-1998. The results demonstrated that good correlation existed between dengue virus NS1 serotype-specific immunoglobulin G (IgG) ELISA and dengue virus plaque reduction neutralization test (PRNT). Our data suggested that NS1 serotype-specific IgG ELISA could replace PRNT for seroepidemiologic studies to differentiate Japanese encephalitis and dengue virus infections and for dengue virus serotyping.
开发了一种NS1血清型特异性间接酶联免疫吸附测定（ELISA），以区分登革病毒的原发性和继发性感染以及原发性登革病毒感染的血清型。在本报告中，我们对1997 - 1998年期间从台湾南部一个孤立岛屿屏东县琉球乡居民采集的血清样本进行了回顾性血清流行病学研究。结果表明，登革病毒NS1血清型特异性免疫球蛋白G（IgG）ELISA与登革病毒蚀斑减少中和试验（PRNT）之间存在良好的相关性。我们的数据表明，NS1血清型特异性IgG ELISA可替代PRNT用于血清流行病学研究，以区分日本脑炎和登革病毒感染以及进行登革病毒血清分型。
