Reymond J F, Grob P J
Vox Sang. 1975;29(5):338-51. doi: 10.1111/j.1423-0410.1975.tb00518.x.
Some biological and physico-chemical properties were tested in 50 lots of transfer factor (TF). Each lot contained the extracts of a pool of 20 buffy coats, each originating from a 400 ml blood donation of a healthy individual. The optical densities at 260 and 280 nm were within a narrow range in all TF lots. The ratio 260/280 was 2.56-3.02. One unit TF (defined as the extract of two buffy coats (corresponding to 1-2 X 10(9) leucocytes) contained 36.58 +/- 3.77 mg of peptides as measured by the Lowry method and 123.6 +/- 4.5 mg ribose as estimated by the orcinol method. Cytotoxicity of TF lots was tested by measurement of chromium release of PHA-stimulated lymphocytes. Cytotoxicity was above 10% in 20 of 27 TF lots tested, most of which were produced by an Amicon ultrafiltration cell. Pyrogenicity was present in 8 of 42 TF lots produced by Amicon filters, but only in 1 of 8 lots produced by Sartorius filters. Sephadex G-10 chromatograms of TF gave characteristic patterns yielding 8 peaks. Sephadex G-25 chromatograms reproducibly showed 5 peaks. The distribution of osmotic activity, peptides and ribose in respect of the chromatographic fractions is given.
对50批转移因子（TF）的一些生物学和物理化学性质进行了测试。每批均包含来自20份血沉棕黄层混合液的提取物，每份血沉棕黄层均源自一名健康个体400毫升的献血。所有TF批次在260和280纳米处的光密度均在狭窄范围内。260/280的比值为2.56 - 3.02。通过Lowry法测定，1个单位的TF（定义为两份血沉棕黄层的提取物（相当于1 - 2×10⁹个白细胞））含有36.58±3.77毫克肽，通过地衣酚法估算含有123.6±4.5毫克核糖。通过测量PHA刺激的淋巴细胞的铬释放来测试TF批次的细胞毒性。在测试的27批TF中，有20批的细胞毒性高于10%，其中大部分是由Amicon超滤细胞生产的。由Amicon过滤器生产的42批TF中有8批有热原性，但由Sartorius过滤器生产的8批中只有1批有热原性。TF的Sephadex G - 10色谱图呈现出有8个峰的特征图谱。Sephadex G - 25色谱图可重复地显示出5个峰。给出了色谱级分中渗透活性、肽和核糖的分布情况。
