Department of Biology, "Alexandru Ioan Cuza" University of Iasi, Iasi, Romania.
J Environ Health Sci Eng. 2014 Jan 8;12(1):15. doi: 10.1186/2052-336X-12-15.
Extremely low frequency electromagnetic fields aren't considered as a real carcinogenic agent despite the fact that some studies have showed impairment of the DNA integrity in different cells lines. The aim of this study was evaluation of the late effects of a 100 Hz and 5.6 mT electromagnetic field, applied continuously or discontinuously, on the DNA integrity of Vero cells assessed by alkaline Comet assay and by cell cycle analysis. Normal Vero cells were exposed to extremely low frequency electromagnetic fields (100 Hz, 5.6 mT) for 45 minutes. The Comet assay and cell cycle analysis were performed 48 hours after the treatment.
Exposed samples presented an increase of the number of cells with high damaged DNA as compared with non-exposed cells. Quantitative evaluation of the comet assay showed a significantly (<0.001) increase of the tail lengths, of the quantity of DNA in tail and of Olive tail moments, respectively. Cell cycle analysis showed an increase of the frequency of the cells in S phase, proving the occurrence of single strand breaks. The most probable mechanism of induction of the registered effects is the production of different types of reactive oxygen species.
The analysis of the registered comet indices and of cell cycle showed that extremely low frequency electromagnetic field of 100 Hz and 5.6 mT had a genotoxic impact on Vero cells.
尽管一些研究表明不同细胞系的 DNA 完整性受损，但极低频电磁场并未被认为是一种真正的致癌剂。本研究旨在通过碱性彗星试验和细胞周期分析评估连续或间断暴露于 100 Hz、5.6 mT 电磁场对 Vero 细胞 DNA 完整性的晚期影响。正常 Vero 细胞暴露于极低频电磁场（100 Hz、5.6 mT）45 分钟。处理后 48 小时进行彗星试验和细胞周期分析。
与未暴露细胞相比，暴露样品中具有高损伤 DNA 的细胞数量增加。彗星试验的定量评估显示，尾长、尾部 DNA 量和 Olive 尾矩分别显著增加（<0.001）。细胞周期分析显示 S 期细胞频率增加，证明发生了单链断裂。登记效应的最可能诱导机制是产生不同类型的活性氧。
对记录的彗星指数和细胞周期的分析表明，100 Hz、5.6 mT 的极低频电磁场对 Vero 细胞具有遗传毒性影响。
