Wang H X, Ng T B
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing.
Life Sci. 2001 Dec 7;70(3):357-65. doi: 10.1016/s0024-3205(01)01399-6.
A novel antifungal protein, designated allivin, was isolated from bulbs of the round-cloved garlic Allium sativum var. round clove with a procedure involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-Sepharose and FPLC-gel filtration on Superdex 75. Allivin possessed an N-terminal sequence demonstrating very little similarity to sequences of Allium sativum chitinases and ribosome inactivating proteins. Allivin exhibited a molecular weight of 13 kDa in gel filtration and SDS-polyacrylamide gel electrophoresis. It displayed antifungal activity against Botrytis cinerea, Mycosphaerella arachidicola and Physalospora piricola. It inhibited translation in a cell-free rabbit reticulocyte system with an IC50 of 1.6 microM.
从圆瓣大蒜（Allium sativum var. round clove）的鳞茎中分离出一种名为蒜杀菌素（allivin）的新型抗真菌蛋白，其分离过程包括在DEAE-纤维素上进行离子交换色谱、在Affi-凝胶蓝胶上进行亲和色谱、在CM-琼脂糖凝胶上进行离子交换色谱以及在Superdex 75上进行快速蛋白质液相色谱-凝胶过滤。蒜杀菌素的N端序列与大蒜几丁质酶和核糖体失活蛋白的序列相似度极低。在凝胶过滤和SDS-聚丙烯酰胺凝胶电泳中，蒜杀菌素的分子量为13 kDa。它对灰葡萄孢（Botrytis cinerea）、落花生球腔菌（Mycosphaerella arachidicola）和梨轮纹病菌（Physalospora piricola）具有抗真菌活性。在无细胞兔网织红细胞系统中，它抑制翻译的IC50为1.6 microM。
