Chu K T, Liu K H, Ng T B
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Peptides. 2003 May;24(5):659-63. doi: 10.1016/s0196-9781(03)00134-7.
A peptide designated cicerarin, with an N-terminal amino acid sequence VKSTGRADDDLAVKTKYLPP dissimilar from known proteins and peptides and a molecular mass of 8kDa, was isolated from seeds of the green chickpea Cicer arietinum cv green chickpea. Cicerarin was isolated with a procedure that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Cicerarin was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel in 10mM Tris-HCl buffer (pH 7.3). Cicerarin exerted antifungal activity against Botrytis cinerea, Mycosphaerella arachidicola, and Physalospora piricola. The antifungal activity was preserved after exposure to 100 degrees C for 15min.
从绿鹰嘴豆（Cicer arietinum cv green chickpea）种子中分离出一种名为鹰嘴豆素（cicerarin）的肽，其N端氨基酸序列为VKSTGRADDDLAVKTKYLPP，与已知蛋白质和肽不同，分子量为8 kDa。鹰嘴豆素的分离过程包括在DEAE-纤维素上进行离子交换色谱、在Affi-gel蓝凝胶上进行亲和色谱以及在Superdex 75上通过快速蛋白质液相色谱进行凝胶过滤。在10 mM Tris-HCl缓冲液（pH 7.3）中，鹰嘴豆素不被DEAE-纤维素吸附，但被Affi-gel蓝凝胶吸附。鹰嘴豆素对灰葡萄孢（Botrytis cinerea）、花生球腔菌（Mycosphaerella arachidicola）和梨孢盘菌（Physalospora piricola）具有抗真菌活性。在100℃下暴露15分钟后，其抗真菌活性得以保留。
