Wang Hexiang, Ng Tzi Bun
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China.
Biochem Biophys Res Commun. 2005 Oct 14;336(1):100-4. doi: 10.1016/j.bbrc.2005.08.058.
There are very few reports on antifungal proteins from rhizomes and there is none from the family of Zingiberaceae. An antifungal protein with a novel N-terminal sequence was isolated from ginger rhizomes utilizing a protocol that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and fast protein liquid chromatography on Superdex 75. The protein was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel. It exhibited an apparent molecular mass of 32kDa and exerted antifungal activity toward various fungi including Botrytis cinerea, Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola.
关于根茎类抗真菌蛋白的报道非常少，姜科植物中尚无此类报道。利用一种包含在DEAE - 纤维素上进行离子交换色谱、在Affi - 凝胶蓝胶上进行亲和色谱以及在Superdex 75上进行快速蛋白质液相色谱的方法，从姜根茎中分离出一种具有新型N端序列的抗真菌蛋白。该蛋白不被DEAE - 纤维素吸附，但能被Affi - 凝胶蓝胶吸附。它的表观分子量为32kDa，对包括灰葡萄孢、尖孢镰刀菌、花生球腔菌和梨孢炭疽菌在内的多种真菌具有抗真菌活性。
