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博伊丁假丝酵母的乙醇氧化酶对甲醛的氧化作用。

Oxidation of formaldehyde by alcohol oxidase of Candida boidinii.

作者信息

Sahm H

出版信息

Arch Microbiol. 1975 Oct 27;105(2):179-81. doi: 10.1007/BF00447134.

DOI:10.1007/BF00447134
PMID:1200740
Abstract

A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the purified alcohol oxidase which oxidizes, besides lower primary alcohols, formaldehyde to formate. The Km value for formaldehyde is 5.7 mM. It seems that alcohol oxidase is not implicated in formaldehyde oxidation in vivo.

摘要

在甲醇培养的博伊丁假丝酵母的无细胞提取物中发现了甲醛氧化酶活性。突变体48中乙醇氧化酶活性的丧失导致甲醛氧化酶活性的丧失，这表明相同的酶可能负责这两种活性。用纯化的乙醇氧化酶可以证明这一点，该酶除了能氧化低级伯醇外，还能将甲醛氧化为甲酸。甲醛的Km值为5.7 mM。似乎乙醇氧化酶在体内与甲醛氧化无关。

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本文引用的文献

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