快速分离分离的肝细胞或类似组织碎片以分析细胞成分。
Rapid separation of isolated hepatocytes or similar tissue fragments for analysis of cell constituents.
作者信息
Hems R, Lund P, Krebs H A
出版信息
Biochem J. 1975 Jul;150(1):47-50. doi: 10.1042/bj1500047.
Abstract
A simple device is described for the rapid centrifugal separation of isolated hepatocytes or similar tissue fragments from the suspending medium. Rapid separation is essential if meaningful information on the concentrations of cell constituents and their distribution between cell and suspension medium is to be obtained. The usefulness of the technique is illustrated by measurements of hepatocyte constituents (glutamate, aspartate, alanine, K+) which show large concentration gradients against the extracellular medium.
摘要
本文描述了一种简单的装置，用于从悬浮介质中快速离心分离分离的肝细胞或类似的组织碎片。如果要获得有关细胞成分浓度及其在细胞与悬浮介质之间分布的有意义信息，快速分离至关重要。通过测量肝细胞成分（谷氨酸、天冬氨酸、丙氨酸、K+）说明了该技术的实用性，这些成分显示出相对于细胞外介质的大浓度梯度。
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本文引用的文献
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