Dunsche Anton, Açil Yahya, Dommisch Henrik, Siebert Reiner, Schröder Jens M, Jepsen Søren
Department of Oral and Maxillofacial Surgery, University Hospital Kiel, Germany.
Eur J Oral Sci. 2002 Apr;110(2):121-4. doi: 10.1034/j.1600-0722.2002.11186.x.
The purpose of this study was to investigate the expression of human beta-defensins (hBD), especially of the recently discovered hBD-3, in oral tissues by reverse-transcription polymerase chain reaction (RT-PCR). Primary oral keratinocytes (n = 3) and fibroblasts (n = 3), 64 non-inflamed and 40 inflamed oral tissue samples, and 10 samples of salivary glands, were examined. The transcripts for hBD-3 (61/64), as well as for hBD-1 (64/64) and hBD-2 (54/64), were found to be widely expressed in non-inflamed oral tissues. In contrast, only 23, 22 and 24 of the 40 inflamed tissues showed detectable hBD-1, -2 and -3 transcripts, respectively. In salivary glands, mRNA expression was constitutive for hBD-1, frequent for hBD-2 (9/10), and infrequent for hBD-3 (4/10). Oral keratinocytes, but not fibroblasts, contained transcripts for all beta-defensins, suggesting that the novel hBD-3 is also produced in the epithelial compartment of oral tissues. The results indicate an important role for the novel hBD-3, as well as for hBD-1 and hBD-2, in the innate oral epithelial host defense.
本研究旨在通过逆转录聚合酶链反应（RT-PCR）研究人β-防御素（hBD），尤其是最近发现的hBD-3在口腔组织中的表达。检测了原代口腔角质形成细胞（n = 3）和成纤维细胞（n = 3）、64份非炎症性和40份炎症性口腔组织样本以及10份唾液腺样本。发现hBD-3（61/64）以及hBD-1（64/64）和hBD-2（54/64）的转录本在非炎症性口腔组织中广泛表达。相比之下，40份炎症性组织中分别只有23、22和24份显示可检测到的hBD-1、-2和-3转录本。在唾液腺中，hBD-1的mRNA表达呈组成性，hBD-2的表达常见（9/10），hBD-3的表达少见（4/10）。口腔角质形成细胞而非成纤维细胞含有所有β-防御素的转录本，这表明新型hBD-3也在口腔组织的上皮部分产生。结果表明新型hBD-3以及hBD-1和hBD-2在口腔上皮先天性宿主防御中起重要作用。
